Comparative pathology of neurovirulent lineage 1 (NY99/385) and lineage 2 (SPU93/01) West Nile virus Infections in BALBc mice

dc.contributor.authorWilliams, June Heather
dc.contributor.authorMentoor, Juliet Lewie Dionee
dc.contributor.authorVan Wilpe, Erna
dc.contributor.authorVenter, Marietjie
dc.contributor.emailjune.williams@up.ac.zaen_ZA
dc.date.accessioned2016-02-09T12:07:52Z
dc.date.available2016-02-09T12:07:52Z
dc.date.issued2015-01
dc.description.abstractThe pathology in mice infected with neurovirulent South African lineage 2 West Nile virus (WNV) strains has not previously been described. Three- to 4-month-old male BALBc mice were infected with South African neurovirulent lineage 2 (SPU93/01) or lineage 1 (NY385/99) WNV strains and the gross and microscopic central nervous system (CNS) and extra-CNS pathology of both investigated and compared. Mice infected with both lineages showed similar illness, paralysis, and death from days 7 to 11 postinfection (PI). Two survivors of each lineage were euthanized on day 21 PI. WNV infection was confirmed by nested real-time reverse transcription polymerase chain reaction of tissues, mostly brain, in the majority of mice euthanized sick or that died and in 1 healthy lineage 2 survivor. Gross lesions caused by both lineages were identical and included marked gastric and proximal small intestinal fluid distension as described in a previous mouse study, but intestinal microscopic lesions differed. CNS lesions were subtle. Immunohistochemical (IHC)–positive labeling for WNV E protein was found in neurons multifocally in the brain of 3 lineage 1–infected and 3 lineage 2–infected mice from days 9 to 11 PI, 4 of these including brainstem neurons, and of cecal myenteric ganglion neurons in 1 lineage 2–infected day 8 PI mouse. Findings supported hypotheses in hamsters that gastrointestinal lesions are likely of brainstem origin. Ultrastructurally, virus-associated cytoplasmic vesicular or crystalline structures, or amorphous structures, were found to label IHC positive in control-positive avian cardiomyocytes and mouse thalamic neurons, respectively, and WNV-like 50-nm particles, which were scarce, did not label.en_ZA
dc.description.librarianhb2015en_ZA
dc.description.sponsorshipNational Research Foundation of South Africa and Pfizer Animal Health. Section of Pathology of the Department of Paraclinical Sciences of the Faculty of Veterinary Science, University of Pretoria, South Africa.en_ZA
dc.description.urihttp://vet.sagepub.comen_ZA
dc.identifier.citationWilliams, JH, Mentoor, JDL, Van Wilpe, E & Venter, M 2015, 'Comparative pathology of neurovirulent lineage 1 (NY99/385) and lineage 2 (SPU93/01) West Nile virus Infections in BALBc mice', Veterinary Pathology, vol. 52, no.1, pp. 140-151.en_ZA
dc.identifier.issn0300-9858 (print)
dc.identifier.issn1544-2217 (online)
dc.identifier.other10.1177/0300985813520246
dc.identifier.urihttp://hdl.handle.net/2263/51292
dc.language.isoenen_ZA
dc.publisherSageen_ZA
dc.rights© The Author(s) 2014en_ZA
dc.subjectBALBc miceen_ZA
dc.subjectHistopathologyen_ZA
dc.subjectImmunohistochemistryen_ZA
dc.subjectNeurovirulenten_ZA
dc.subjectPathologyen_ZA
dc.subjectUltrastructureen_ZA
dc.subjectWest Nile virus (WNV)en_ZA
dc.subjectWest Nile virus lineages 1en_ZA
dc.subjectWest Nile virus lineages 2en_ZA
dc.subjectWNV
dc.titleComparative pathology of neurovirulent lineage 1 (NY99/385) and lineage 2 (SPU93/01) West Nile virus Infections in BALBc miceen_ZA
dc.typePostprint Articleen_ZA

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