Comparative pathology of neurovirulent lineage 1 (NY99/385) and lineage 2 (SPU93/01) West Nile virus Infections in BALBc mice
dc.contributor.author | Williams, June Heather | |
dc.contributor.author | Mentoor, Juliet Lewie Dionee | |
dc.contributor.author | Van Wilpe, Erna | |
dc.contributor.author | Venter, Marietjie | |
dc.contributor.email | june.williams@up.ac.za | en_ZA |
dc.date.accessioned | 2016-02-09T12:07:52Z | |
dc.date.available | 2016-02-09T12:07:52Z | |
dc.date.issued | 2015-01 | |
dc.description.abstract | The pathology in mice infected with neurovirulent South African lineage 2 West Nile virus (WNV) strains has not previously been described. Three- to 4-month-old male BALBc mice were infected with South African neurovirulent lineage 2 (SPU93/01) or lineage 1 (NY385/99) WNV strains and the gross and microscopic central nervous system (CNS) and extra-CNS pathology of both investigated and compared. Mice infected with both lineages showed similar illness, paralysis, and death from days 7 to 11 postinfection (PI). Two survivors of each lineage were euthanized on day 21 PI. WNV infection was confirmed by nested real-time reverse transcription polymerase chain reaction of tissues, mostly brain, in the majority of mice euthanized sick or that died and in 1 healthy lineage 2 survivor. Gross lesions caused by both lineages were identical and included marked gastric and proximal small intestinal fluid distension as described in a previous mouse study, but intestinal microscopic lesions differed. CNS lesions were subtle. Immunohistochemical (IHC)–positive labeling for WNV E protein was found in neurons multifocally in the brain of 3 lineage 1–infected and 3 lineage 2–infected mice from days 9 to 11 PI, 4 of these including brainstem neurons, and of cecal myenteric ganglion neurons in 1 lineage 2–infected day 8 PI mouse. Findings supported hypotheses in hamsters that gastrointestinal lesions are likely of brainstem origin. Ultrastructurally, virus-associated cytoplasmic vesicular or crystalline structures, or amorphous structures, were found to label IHC positive in control-positive avian cardiomyocytes and mouse thalamic neurons, respectively, and WNV-like 50-nm particles, which were scarce, did not label. | en_ZA |
dc.description.librarian | hb2015 | en_ZA |
dc.description.sponsorship | National Research Foundation of South Africa and Pfizer Animal Health. Section of Pathology of the Department of Paraclinical Sciences of the Faculty of Veterinary Science, University of Pretoria, South Africa. | en_ZA |
dc.description.uri | http://vet.sagepub.com | en_ZA |
dc.identifier.citation | Williams, JH, Mentoor, JDL, Van Wilpe, E & Venter, M 2015, 'Comparative pathology of neurovirulent lineage 1 (NY99/385) and lineage 2 (SPU93/01) West Nile virus Infections in BALBc mice', Veterinary Pathology, vol. 52, no.1, pp. 140-151. | en_ZA |
dc.identifier.issn | 0300-9858 (print) | |
dc.identifier.issn | 1544-2217 (online) | |
dc.identifier.other | 10.1177/0300985813520246 | |
dc.identifier.uri | http://hdl.handle.net/2263/51292 | |
dc.language.iso | en | en_ZA |
dc.publisher | Sage | en_ZA |
dc.rights | © The Author(s) 2014 | en_ZA |
dc.subject | BALBc mice | en_ZA |
dc.subject | Histopathology | en_ZA |
dc.subject | Immunohistochemistry | en_ZA |
dc.subject | Neurovirulent | en_ZA |
dc.subject | Pathology | en_ZA |
dc.subject | Ultrastructure | en_ZA |
dc.subject | West Nile virus (WNV) | en_ZA |
dc.subject | West Nile virus lineages 1 | en_ZA |
dc.subject | West Nile virus lineages 2 | en_ZA |
dc.subject | WNV | |
dc.title | Comparative pathology of neurovirulent lineage 1 (NY99/385) and lineage 2 (SPU93/01) West Nile virus Infections in BALBc mice | en_ZA |
dc.type | Postprint Article | en_ZA |