Cryptosporidium genotypes in children and calves living at the wildlife or livestock interface of the Kruger National Park, South Africa

dc.contributor.authorAbu Samra, Nada
dc.contributor.authorJori, Ferran J.
dc.contributor.authorCaccio, Simone M.
dc.contributor.authorFrean, John
dc.contributor.authorPoonsamy, Bhavani
dc.contributor.authorThompson, P.N. (Peter N.)
dc.date.accessioned2016-06-15T07:17:12Z
dc.date.available2016-06-15T07:17:12Z
dc.date.issued2016-05-20
dc.description.abstractCryptosporidium infection is one of the most common causes of parasitic diarrhoea worldwide in cattle and humans. In developing countries, human cryptosporidiosis is most prevalent during early childhood and links between zoonotic infection and animal related activities have been demonstrated. This study investigated the prevalence and species/genotype distribution of Cryptosporidium among children (< 5 years) and calves (< 6 months) living in a rural farming area adjacent to the Kruger National Park in South Africa, where interactions between humans and wild and domestic animals are known to occur. Cryptosporidium oocysts were detected in 8/143 stool samples of children recruited within the hospital system (5.6%; 95% CI 2.4%, 10.7%) and in 2/352 faecal samples of calves (0.6%; 95% CI 0.1%, 2.0%) using the modified Ziehl–Neelsen (MZN) staining technique. Microscopy positive samples from children were further analysed by PCR targeting the 18S rRNA gene and identified as Cryptosporidium hominis (3/4) and Cryptosporidium meleagridis (1/4). Regardless of the microscopy outcome, randomly selected samples (n = 36) from calves 0–4 months of age were amplified and sequenced at the 18S rRNA gene using nested PCR. Two calves tested positive (5.6%; 95% CI 1.7%, 18.7%), and revealed the presence of Cryptosporidium parvum and Cryptosporidium bovis. The detection of only two zoonotic species (C. parvum in one calf and C. meleagridis in one child) suggests that zoonotic cryptosporidiosis is not currently widespread in our study area; however, the potential exists for amplification of transmission in an immunocompromised population.en_ZA
dc.description.departmentProduction Animal Studiesen_ZA
dc.description.librarianam2016en_ZA
dc.description.sponsorshipThe Faculty of Veterinary Science, University of Pretoriaen_ZA
dc.description.urihttp://www.ojvr.orgen_ZA
dc.identifier.citationAbu Samra, N., Jori, F., Cacciò, S.M., Frean, J., Poonsamy, B. & Thompson, P.N., 2016, ‘Cryptosporidium genotypes in children and calves living at the wildlife or livestock interface of the Kruger National Park, South Africa’, Onderstepoort Journal of Veterinary Research 83(1), a1024. http://dx.DOI.org/ 10.4102/ojvr.v83i1.1024.en_ZA
dc.identifier.issn0030-2465 (print)
dc.identifier.issn2219-0635 (online)
dc.identifier.other10.4102/ojvr.v83i1.1024
dc.identifier.other7403220452
dc.identifier.otherJ-2534-2013
dc.identifier.urihttp://hdl.handle.net/2263/53232
dc.language.isoenen_ZA
dc.publisherAOSIS OpenJournalsen_ZA
dc.rights© 2016. The Authors. Licensee: AOSIS. This work is licensed under the Creative Commons Attribution License.en_ZA
dc.subjectChildrenen_ZA
dc.subjectCattleen_ZA
dc.subjectCryptosporidium infectionen_ZA
dc.subjectKruger National Park (South Africa)en_ZA
dc.subject.otherVeterinary science articles SDG-03en_ZA
dc.subject.otherVeterinary science articles SDG-11en_ZA
dc.subject.otherSDG-03: Good health and well-being
dc.subject.otherSDG-11: Sustainable cities and communities
dc.titleCryptosporidium genotypes in children and calves living at the wildlife or livestock interface of the Kruger National Park, South Africaen_ZA
dc.typeArticleen_ZA

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