Diagnostic accuracy of a duplex real-time reverse transcription quantitative PCR assay for detection of African horse sickness virus

dc.contributor.authorGuthrie, Alan John
dc.contributor.authorMacLachlan, N.J. (James)
dc.contributor.authorJoone, Christopher
dc.contributor.authorLourens, Carina W.
dc.contributor.authorWeyer, Camilla Theresa
dc.contributor.authorQuan, Melvyn
dc.contributor.authorMonyai, Mpho S.
dc.contributor.authorGardner, Ian A.
dc.contributor.emailalan.guthrie@up.ac.zaen
dc.date.accessioned2014-05-21T10:20:06Z
dc.date.available2014-05-21T10:20:06Z
dc.date.issued2013
dc.description.abstractBlood samples collected from 503 suspect cases of African horse sickness (AHS) and another 503 from uninfected, unvaccinated South African horses, as well as 98 samples from horses from an AHS free country, were tested with an AHS virus (AHSV) specific duplex real-time reverse transcription quantitative PCR (RT-qPCR) assay and virus isolation (VI). The diagnostic sensitivity and specificity of this AHSV RTqPCR assay and VI were estimated using a 2-test 2-population Bayesian latent class model which made no assumptions about the true infection status of the tested animals and allowed for the possibility of conditional dependence (correlation) in test results. Median diagnostic sensitivity and specificity of the AHSV RT-qPCR were 97.8% and 99.9%, respectively. Median diagnostic specificity of virus isolation was >99% whereas the estimated diagnostic sensitivity was 44.2%. The AHSV RT-qPCR assay provides for rapid, high-throughput analysis of samples, and is both analytically and diagnostically sensitive and specific. This assay is potentially highly useful for demonstrating freedom or infection of horses with AHSV, thus it is appropriate that its reproducibility be evaluated in other laboratories as a global standard for detection of AHSV.en_US
dc.description.librarianhb2014en
dc.description.librarianab2014
dc.description.sponsorshipRacing South Africa (Pty) Ltd., The Mary Slack and Daughters Foundation and Thoroughbred Racing Trust of South Africaen
dc.description.urihttp://www.elsevier.com/locate/jvirometen
dc.identifier.citationGuthrie, AJ, MacLachlan, NJ, Joone, C, Lourens, CW, Weyer, CT, Melvyn Q, Monyai, MS & Gardner, IA 2013, 'Diagnostic accuracy of a duplex real-time reverse transcription quantitative PCR assay for detection of African horse sickness virus', Journal of Virological Methods, vol. 189, no. 1, pp. 30-35.en
dc.identifier.issn0166-0934 (print)
dc.identifier.issn1879-0984 (online)
dc.identifier.other10.1016/j.jviromet.2012.12.014
dc.identifier.other24338988000
dc.identifier.otherN-9316-2014
dc.identifier.other0000-0003-3352-8488
dc.identifier.other7006535470
dc.identifier.otherJ-6375-2013
dc.identifier.other0000-0001-7729-9918
dc.identifier.urihttp://hdl.handle.net/2263/39837
dc.language.isoenen
dc.publisherElsevieren
dc.rights© 2012 Elsevier B.V. All rights reserved.Notice : this is the author’s version of a work that was accepted for publication in Journal of Virological Methods. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Virological Methods, vol. 189, no.1, pp.30-35, 2013. doi : 10.1016/j.jviromet.2012.12.014en
dc.subjectDiagnostic sensitivityen
dc.subjectDiagnostic specificityen
dc.subjectRT-qPCRen
dc.subjectAHSVen
dc.subjectStandards for Reporting of Diagnostic Accuracy (STARD)en
dc.subject.lcshAfrican horse sickness virusen
dc.subject.lcshPolymerase chain reactionen
dc.titleDiagnostic accuracy of a duplex real-time reverse transcription quantitative PCR assay for detection of African horse sickness virusen
dc.typePostprint Articleen

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Guthrie_Diagnostic_2013.pdf
Size:
3.09 MB
Format:
Adobe Portable Document Format
Description:
Postprint Article

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description: