Molecular evolution of Klebsiella pneumoniae ST278 harboring blaNDM-7 involved in nosocomial transmission

dc.contributor.authorLynch, T.
dc.contributor.authorChen, L.
dc.contributor.authorPeirano, Gisele
dc.contributor.authorGregson, D.B.
dc.contributor.authorChurch, D.L.
dc.contributor.authorConly, J.
dc.contributor.authorKreiswirth, B.N.
dc.contributor.authorPitout, Johann D.D.
dc.date.accessioned2016-06-24T06:20:20Z
dc.date.issued2016-06
dc.description.abstractDuring 2013, ST278 Klebsiella pneumoniae with blaNDM-7 was isolated from the urine (KpN01) and rectum (KpN02) of a patient in Calgary, Canada. The same strain (KpN04) was subsequently isolated from another patient in the same unit. Interestingly, a carbapenem-susceptible ST278 (KpN06) was obtained one month later from the blood of the second patient. Next generation sequencing (NGS) revealed that the loss of carbapenem-resistance in KpN06 was due to a 5-kb deletion on the blaNDM-7-harboring IncX3 plasmid. In addition, an IncFIB plasmid in KpN06 had a 27 kb deletion that removed genes encoding for heavy metal resistance. Phylogenetic analysis showed that the ST278 K. pneumoniae from patient 2 were likely descendants of KpN02 and that KpN06 was a close progenitor of an environmental ST278. It is unclear whether KpN06 lost the blaNDM-7 gene in vivo. This study detailed the remarkable plasticity and speed of evolutionary changes in multidrug resistance K. pneumoniae demonstrating the highly recombinant nature of this species. It also highlights the ability of NGS to clarify molecular micro-evolutionary events within antibiotic-resistant organisms.en_ZA
dc.description.departmentMedical Microbiologyen_ZA
dc.description.embargo2017-06-30
dc.description.librarianhb2016en_ZA
dc.description.sponsorshipThis work was supported in part by a research grant from the Calgary Laboratory Services (#10009392) and grants from the National Institutes of Health (1R01AI090155 to B.N.K, and R21AI117338 to L.C).en_ZA
dc.description.urihttp://jid.oxfordjournals.orgen_ZA
dc.identifier.citationLynch, T, Chen, L, Peirano, G, Gregson, DB, Church, DL, Conly, J, Kreiswirth, BN & Pitout, JDD 2016, 'Molecular evolution of Klebsiella pneumoniae ST278 harboring blaNDM-7 involved in nosocomial transmission', Journal of Infectious Diseases, vol. 214, no. 5, pp. 798-806.en_ZA
dc.identifier.issn0022-1899 (print)
dc.identifier.issn1537-6613 (online)
dc.identifier.other10.1093/infdis/jiw240
dc.identifier.urihttp://hdl.handle.net/2263/53383
dc.language.isoenen_ZA
dc.publisherOxford University Pressen_ZA
dc.rights© 2016 by the Infectious Diseases Society of America. All rights reserved. This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Journal of Infectious Diseases following peer review. The definitive publisher-authenticated version is : Molecular evolution of Klebsiella pneumoniae ST278 harboring blaNDM-7 involved in nosocomial transmission, Journal of Infectious Diseases, vol. 214, no. 5, pp. 798-806, 2016. doi : 10.1093/infdis/jiw240, is available online at : http://jid.oxfordjournals.org.en_ZA
dc.subjectK. pneumoniaeen_ZA
dc.subjectST278en_ZA
dc.subjectCarbapenemasesen_ZA
dc.subjectblaNDM-7en_ZA
dc.subjectPlasmiden_ZA
dc.subjectMicro-evolutionen_ZA
dc.titleMolecular evolution of Klebsiella pneumoniae ST278 harboring blaNDM-7 involved in nosocomial transmissionen_ZA
dc.typePostprint Articleen_ZA

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