Evidence for multidrug resistance in nonpathogenic mycoplasma species isolated from South African poultry

dc.contributor.authorBeylefeld, Amanda
dc.contributor.authorWambulawaye, Pamela
dc.contributor.authorBwala, Dauda Garba
dc.contributor.authorGouws, Johan
dc.contributor.authorLukhele, Obed Mooki
dc.contributor.authorWandrag, D.B.R. (Daniel)
dc.contributor.authorAbolnik, Celia
dc.date.accessioned2019-10-15T07:16:53Z
dc.date.available2019-10-15T07:16:53Z
dc.date.issued2018-11
dc.description.abstractOne hundred seventy-eight mycoplasma strains isolated from South African poultry flocks between 2003 and 2015 were identified by full-genome sequencing and phylogenetic analysis of the 16S rRNA gene and were classified as follows: Mycoplasma gallisepticum (25%), M. gallinarum (25%), M. gallinaceum, (23%), M. pullorum (14%), M. synoviae (10%), and M. iners (3%), as well as one Acheoplasma laidlawii strain (1%). MIC testing was performed on the axenic samples, and numerous strains of each species were resistant to either chlortetracycline or tylosin or both, with variable sensitivity to enrofloxacin. The strains of all species tested remained sensitive to tiamulin, except for one M. gallinaceum sample that demonstrated intermediate sensitivity. The mutation of A to G at position 2059 (A2059G) in the 23S rRNA gene, which is associated with macrolide resistance, was found in the South African M. gallisepticum and M. synoviae strains, as well as a clear correlation between macrolide resistance in M. gallinarum and M. gallinaceum and mutations G354A and G748A in the L4 ribosomal protein and 23S rRNA gene, respectively. No correlation between resistance and point mutations in the genes studied could be found for M. pullorum. Only a few strains were resistant to enrofloxacin, apart from one M. synoviae strain with point mutation D420N, which has been associated with quinolone resistance, and no other known markers for quinolone resistance were found in this study. Proportionally more antimicrobial-resistant strains were detected in M. gallinaceum, M. gallinarum, and M. pullorum than in M. gallisepticum and M. synoviae. Of concern, three M. gallinaceum strains showed multidrug resistance to chlortetracycline, tylosin, and oxytetracycline.en_ZA
dc.description.departmentProduction Animal Studiesen_ZA
dc.description.departmentVeterinary Tropical Diseasesen_ZA
dc.description.librarianam2019en_ZA
dc.description.sponsorshipThe Technology Innovation Agency-Tshwane Animal Health Innovation Cluster Initiative (grant number TAHC12-00034). A.B. was funded by the Health and Welfare Sector Education and Training Authority, University of Pretoria, and the National Research Foundation.en_ZA
dc.description.urihttp://aem.asm.orgen_ZA
dc.identifier.citationBeylefeld A, Wambulawaye P, Bwala DG, Gouws JJ, Lukhele OM, Wandrag DBR, Abolnik C. 2018. Evidence for multidrug resistance in nonpathogenic Mycoplasma species isolated from South African poultry. Appl Environ Microbiol 84:e01660-18. https://DOI.org/10.1128/AEM.01660-18.en_ZA
dc.identifier.issn0099-2240 (print)
dc.identifier.issn1098-5336 (online)
dc.identifier.other10.1128/AEM.01660-18
dc.identifier.urihttp://hdl.handle.net/2263/71820
dc.language.isoenen_ZA
dc.publisherAmerican Society for Microbiologyen_ZA
dc.rights© 2019 American Society for Microbiologyen_ZA
dc.subjectMycoplasmaen_ZA
dc.subjectMICen_ZA
dc.subjectPoultryen_ZA
dc.subject16S rRNAen_ZA
dc.subjectAntibiotic resistanceen_ZA
dc.subject23S rRNAen_ZA
dc.subjectDNA gyraseen_ZA
dc.subjectDNA topoisomeraseen_ZA
dc.titleEvidence for multidrug resistance in nonpathogenic mycoplasma species isolated from South African poultryen_ZA
dc.typeArticleen_ZA

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Beylefeld_Evidence_2018.pdf
Size:
871.78 KB
Format:
Adobe Portable Document Format
Description:
Article

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description: