Exploiting Pan Influenza A and Pan Influenza B pseudotype libraries for efficient vaccine antigen selection

dc.contributor.authorDel Rosario, Joanne Marie M.
dc.contributor.authorDa Costa, Kelly A.S.
dc.contributor.authorAsbach, Benedikt
dc.contributor.authorFerrara, Francesca
dc.contributor.authorFerrari, Matteo
dc.contributor.authorWells, David A.
dc.contributor.authorMann, Gurdip Singh
dc.contributor.authorAmeh, Veronica Odinya
dc.contributor.authorSabeta, Claude Taurai
dc.contributor.authorBanyard, Ashley C.
dc.contributor.authorKinsley, Rebecca
dc.contributor.authorScott, Simon D.
dc.contributor.authorWagner, Ralf
dc.contributor.authorHeeney, Jonathan L.
dc.contributor.authorCarnell, George W.
dc.contributor.authorTemperton, Nigel J.
dc.date.accessioned2022-04-29T13:21:23Z
dc.date.available2022-04-29T13:21:23Z
dc.date.issued2021-07
dc.description.abstractWe developed an influenza hemagglutinin (HA) pseudotype library encompassing Influenza A subtypes HA1-18 and Influenza B subtypes (both lineages) to be employed in influenza pseudotype microneutralization (pMN) assays. The pMN is highly sensitive and specific for detecting virus-specific neutralizing antibodies against influenza viruses and can be used to assess antibody functionality in vitro. Here we show the production of these viral HA pseudotypes and their employment as substitutes for wildtype viruses in influenza neutralization assays. We demonstrate their utility in detecting serum responses to vaccination with the ability to evaluate cross-subtype neutralizing responses elicited by specific vaccinating antigens. Our findings may inform further preclinical studies involving immunization dosing regimens in mice and may help in the creation and selection of better antigens for vaccine design. These HA pseudotypes can be harnessed to meet strategic objectives that contribute to the strengthening of global influenza surveillance, expansion of seasonal influenza prevention and control policies, and strengthening pandemic preparedness and response.en_US
dc.description.departmentVeterinary Tropical Diseasesen_US
dc.description.librarianpm2022en_US
dc.description.sponsorshipBill and Melinda Gates Foundation: Grand Challenges Universal Influenza Vaccines Award; UK Research and Innovation (UKRI); EC FETopen (Virofight, Grant 899619); Department of Science and Technology of South Africa; UK Department for the Environment, Food and Rural Affairs (Defra); Scottish and Welsh governments.en_US
dc.description.urihttp://www.mdpi.com/journal/vaccinesen_US
dc.identifier.citationDel Rosario, J.M.M.; da Costa, K.A.S.; Asbach, B.; Ferrara, F.; Ferrari, M.; Wells, D.A.; Mann, G.S.; Ameh, V.O.; Sabeta, C.T.; Banyard, A.C.; et al. Exploiting Pan Influenza A and Pan Influenza B Pseudotype Libraries for Efficient Vaccine Antigen Selection. Vaccines 2021, 9, 741. https://doi.org/10.3390/vaccines9070741.en_US
dc.identifier.issn2076-393X (online)
dc.identifier.other10.3390/ vaccines9070741
dc.identifier.urihttps://repository.up.ac.za/handle/2263/84990
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rights© 2021 by the authors. Licensee: MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en_US
dc.subjectInfluenzaen_US
dc.subjectHemagglutininen_US
dc.subjectPseudotypeen_US
dc.subjectVaccineen_US
dc.subjectImmunogenicityen_US
dc.subjectMonoclonal antibodyen_US
dc.subjectNeutralizationen_US
dc.titleExploiting Pan Influenza A and Pan Influenza B pseudotype libraries for efficient vaccine antigen selectionen_US
dc.typeArticleen_US

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