Clonality and limited population diversity of Fusarium circinatum in Colombia

dc.contributor.authorCeriani, Callin
dc.contributor.authorWingfield, Michael J.
dc.contributor.authorFru, Felix Fon
dc.contributor.authorVan Wyk, S.
dc.contributor.authorRodas, Carlos A.
dc.contributor.authorWingfield, Brenda D.
dc.contributor.authorSteenkamp, Emma Theodora
dc.contributor.emailemma.steenkamp@fabi.up.ac.zaen_US
dc.date.accessioned2024-10-22T12:40:08Z
dc.date.available2024-10-22T12:40:08Z
dc.date.issued2024-06
dc.descriptionDATA AVAILABILITY STATEMENT : The data that support the findings of this study are available from the corresponding author upon reasonable request.en_US
dc.description.abstractFusarium circinatum is an important fungal pathogen of Pinus species utilized in commercial forestry worldwide. In Colombia, it was first found on nursery plants and later in plantations associated with basal cankers on young trees. In this study, we explored the population diversity of the pathogen in Colombia by analyzing 136 isolates collected from diseased nursery plants (2005–2007) and plantation trees (2017 and 2020). These were sourced from different geographical regions and Pinus species. Genotyping was performed using 10 microsatellite markers, while mating types were identified with PCRs targeting the MAT1 locus. Using microsatellites, a total of 33 multilocus haplotypes were detected. Genetic diversity indices showed low levels of diversity in both the overall collection and in specific collection groupings. The data also suggested that a small number of isolates had unique origins in the country (p < .05), and relatively low levels of population differentiation were detected between the nursery and plantation collections. All the isolates were scored as having the MAT1-1 mating type, and no evidence for the random association among microsatellite alleles (p = .0001) was found. Overall, these data suggest that F. circinatum was introduced into Colombia a small number of times, likely on seed for nursery production. Furthermore, the data also indicate that the pathogen has spread from nurseries to the plantations via asexual reproduction and on asymptomatic plants. This has resulted in a highly clonal F. circinatum population in Colombia that has resulted from accidental introductions of the pathogen into a production nursery.en_US
dc.description.departmentBiochemistry, Genetics and Microbiology (BGM)en_US
dc.description.departmentForestry and Agricultural Biotechnology Institute (FABI)en_US
dc.description.librarianhj2024en_US
dc.description.sdgSDG-15:Life on landen_US
dc.description.sponsorshipDepartment of Science and Innovation, South Africa; National Research Foundation; South African Research Chairs Initiative.en_US
dc.description.urihttp://www.wileyonlinelibrary.com/journal/efpen_US
dc.identifier.citationCeriani, C., Wingfield, M. J., Fru, F., van Wyk, S., Rodas, C., Wingfield, B. D., & Steenkamp, E. T. (2024). Clonality and limited population diversity of Fusarium circinatum in Colombia. Forest Pathology, 54, e12864. https://doi.org/10.1111/efp.12864.en_US
dc.identifier.issn1437-4781 (print)
dc.identifier.issn1439-0329 (online)
dc.identifier.other10.1111/efp.12864
dc.identifier.urihttp://hdl.handle.net/2263/98713
dc.language.isoenen_US
dc.publisherWileyen_US
dc.rights© 2024 The Authors. Forest Pathology published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License.en_US
dc.subjectClonalityen_US
dc.subjectMating typeen_US
dc.subjectMicrosatellitesen_US
dc.subjectPine pitch cankeren_US
dc.subjectPopulation structureen_US
dc.subjectReproductive modeen_US
dc.subjectSouth Americaen_US
dc.subjectSDG-15: Life on landen_US
dc.titleClonality and limited population diversity of Fusarium circinatum in Colombiaen_US
dc.typeArticleen_US

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