Optimization of a foot-and-mouth disease virus Southern African Territories–specific solid-phase competitive ELISA for small ruminant serum samples

dc.contributor.authorSeoke, LaToya
dc.contributor.authorFosgate, Geoffrey Theodore
dc.contributor.authorOpperman, Pamela Anne
dc.contributor.authorMalesa, Refiloe P.
dc.contributor.authorLazarus, David Dazhia
dc.contributor.authorSirdar, Mohamed Mahmoud
dc.contributor.authorHeath, Livio
dc.contributor.emailu13077628@tuks.co.zaen_US
dc.date.accessioned2024-02-29T04:45:28Z
dc.date.available2024-02-29T04:45:28Z
dc.date.issued2024-03
dc.description.abstractWe optimized and verified a single-spot solid-phase competitive ELISA (ss-SPCE) to detect antibodies against structural proteins of Southern African Territories (SAT) serotypes of foot-and-mouth disease virus (FMDV) in small ruminants. Sera from goats vaccinated and experimentally challenged with a SAT1 FMDV pool were tested in duplicate at 4 dilutions (1:10, 1:15, 1:22.5, 1:33.8) to optimize the assay. To assess the performance of the assay in naturally infected animals, we evaluated 316 goat and sheep field sera collected during active SAT2 outbreaks. Relative to results of the virus neutralization test, the optimal serum dilution and cutoff percentage inhibition (PI) were 1:15 and 50%, respectively. At these values, the Spearman rank correlation coefficient was 0.85 (p < 0.001), and the sensitivity and specificity (95% CI) were 80.3% (72.6, 87.2) and 91.1% (84.1, 95.9), respectively. Relative to the liquid-phase blocking ELISA and the nonstructural protein ELISA, the ss-SPCE exhibited divergent performance characteristics between the goat and sheep field sera. Repeatability was better for goats, but the correlation and agreement among all 3 assays were better for the sheep sera. The prevalence of SAT2 FMDV infection in the sampled sheep was 23.6%; sampled goats were seemingly FMDV-free. The ss-SPCE is an appropriate FMDV detection tool to investigate the role of small ruminants in the epidemiology of FMD in Africa.en_US
dc.description.departmentProduction Animal Studiesen_US
dc.description.librarianhj2024en_US
dc.description.sdgSDG-03:Good heatlh and well-beingen_US
dc.description.sponsorshipThe Red Meat Research Development (RMRD) of South Africa.en_US
dc.description.urihttps://journals.sagepub.com/home/VDIen_US
dc.identifier.citationSeoke, L., Fosgate, G.T., Opperman, P.A.,, et al. Optimization of a foot-and-mouth disease virus Southern African Territories–specific solid-phase competitive ELISA for small ruminant serum samples. Journal of Veterinary Diagnostic Investigation. 2024; 36(2): 192-204. doi: 10.1177/10406387231218202.en_US
dc.identifier.issn1040-6387 (print)
dc.identifier.issn1943-4936 (online)
dc.identifier.other10.1177/10406387231218202
dc.identifier.urihttp://hdl.handle.net/2263/94976
dc.language.isoenen_US
dc.publisherSageen_US
dc.rights© 2023 The Author(s).en_US
dc.subjectSingle-spot solid-phase competitive ELISAen_US
dc.subjectSouthern African territories (SAT)en_US
dc.subjectFoot-and-mouth disease virus (FMDV)en_US
dc.subjectSmall ruminantsen_US
dc.subjectSDG-03: Good health and well-beingen_US
dc.subjectSerologyen_US
dc.subjectTest optimizationen_US
dc.titleOptimization of a foot-and-mouth disease virus Southern African Territories–specific solid-phase competitive ELISA for small ruminant serum samplesen_US
dc.typePostprint Articleen_US

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