The beta-2-adrenoreceptor agonists, formoterol and indacaterol, but not salbutamol, effectively suppress the reactivity of human neutrophils in vitro

dc.contributor.authorAnderson, Ronald
dc.contributor.authorTheron, Annette J.
dc.contributor.authorSteel, Helen Carolyn
dc.contributor.authorDurandt, Chrisna
dc.contributor.authorTintinger, Gregory Ronald
dc.contributor.authorFeldman, Charles
dc.date.accessioned2014-05-22T09:18:52Z
dc.date.available2014-05-22T09:18:52Z
dc.date.issued2014-03
dc.description.abstractThe clinical relevance of the anti-inflammatory properties of beta-2 agonists remains contentious possibly due to differences in their molecular structures and agonist activities. The current study has compared the effects of 3 different categories of β2-agonists, namely, salbutamol (short-acting), formoterol (long-acting) and indacaterol (ultra-long-acting), at concentrations of 1–1000 nM, with human blood neutrophils in vitro. Neutrophils were activated with either N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP, 1 µM) or platelet-activating factor (PAF, 200 nM) in the absence and presence of the β2-agonists followed by measurement of the generation of reactive oxygen species and leukotriene B4, release of elastase, and expression of the β2-integrin, CR3, using a combination of chemiluminescence, ELISA, colorimetric, and flow cytometric procedures respectively. These were correlated with alterations in the concentrations of intracellular cyclic-AMP and cytosolic Ca2+. At the concentrations tested, formoterol and indacaterol caused equivalent, significant (P<0.05 at 1–10 nM) dose-related inhibition of all of the pro-inflammatory activities tested, while salbutamol was much less effective (P<0.05 at 100 nM and higher). Suppression of neutrophil reactivity was accompanied by elevations in intracellular cAMP and accelerated clearance of Ca2+ from the cytosol of activated neutrophils. These findings demonstrate that β2-agonists vary with respect to their suppressive effects on activated neutrophils.en_US
dc.description.librarianhb2014en_US
dc.description.sponsorshipMedical Research Council and National Research Foundation of South Africaen_US
dc.description.urihttp://www.hindawi.com/journals/mi/en_US
dc.identifier.citationAnderson, R, Theron, AJ, Steel, HC, Durandt, C, Tintinger, GR & Feldman, C 2014,'The beta-2-adrenoreceptor agonists, formoterol and indacaterol, but not salbutamol, effectively suppress the reactivity of human neutrophils in vitro', Mediators of Inflammation, vol.2014, art. 105420, pp. 1-9.en_US
dc.identifier.issn0962-9351 (print)
dc.identifier.issn1466-1861(online)
dc.identifier.otherhttp://dx.doi.org/10.1155/2014/105420
dc.identifier.urihttp://hdl.handle.net/2263/39852
dc.language.isoenen_US
dc.publisherHindawi Publishing Corporationen_US
dc.rights© 2014 Ronald Anderson et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.subjectBeta-2-adrenoreceptor agonistsen_US
dc.subjectFormoterolen_US
dc.subjectIndacaterolen_US
dc.subjectNot salbutamolen_US
dc.subjectReactivity of human neutrophilsen_US
dc.subjectIn vitroen_US
dc.titleThe beta-2-adrenoreceptor agonists, formoterol and indacaterol, but not salbutamol, effectively suppress the reactivity of human neutrophils in vitroen_US
dc.typeArticleen_US

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