Comparison of the survival on ice of thawed Theileria parva sporozoites of different stocks cryoprotected by glycerol or sucrose

dc.contributor.authorMbao, V.
dc.contributor.authorBerkvens, Dirk
dc.contributor.authorDorny, Pierre
dc.contributor.authorVan den Bossche, Peter
dc.contributor.authorMarcotty, Tanguy
dc.date.accessioned2008-05-08T10:44:08Z
dc.date.available2008-05-08T10:44:08Z
dc.date.issued2007-03
dc.description.abstractStabilates of Theileria parva sporozoites are mostly delivered in liquid nitrogen tanks to the East Coast fever immunization points. Using an in vitro titration model, we assessed the loss of infectivity of several stabilates when they are stored in ice baths for up to 24 h. Comparisons, with respect to rates of loss of infectivity, were made between T. parva stocks (Chitongo and Katete), cryoprotectants (sucrose and glycerol) and method of assessment (in vivo and in vitro techniques). Chitongo and Katete stabilates showed similar loss dynamics. The losses were 1–4 % (depending on parasite stock) and 3 % per hour of storage for glycerol and sucrose stabilates respectively, and the loss rates were not significantly different. The results suggest that Chitongo stabilates and sucrose cryoprotected suspensions can be delivered on ice as is done for Katete.en
dc.format.extent257230 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.citationMbao, V, Berkvens, D, Dorny, P, Van Den Bossche, P & Marcotty, T 2007, 'Comparison of the survival on ice of thawed Theileria parva sporozoites of different stocks cryoprotected by glycerol or sucrose', Onderstepoort Journal of Veterinary Research, vol. 74, no. 1, pp. 9-15. [http://www.journals.co.za/ej/ejour_opvet.html]en
dc.identifier.issn0030-2465
dc.identifier.urihttp://hdl.handle.net/2263/5195
dc.language.isoenen
dc.publisherAgricultural Research Council, ARC-OVI and the University of Pretoriaen
dc.rightsAgricultural Research Council, ARC-OVI and the University of Pretoriaen
dc.subjectCold-chainen
dc.subjectImmunizationen
dc.subjectIn vitroen
dc.subjectSporozoitesen
dc.subjectZambiaen
dc.subject.lcshTheileria parvaen
dc.titleComparison of the survival on ice of thawed Theileria parva sporozoites of different stocks cryoprotected by glycerol or sucroseen
dc.typeArticleen

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