Design of oleanolic acid-based hybrid compounds as potential pharmaceutical scaffolds
Loading...
Date
Authors
Khwaza, Vuyolwethu
Oyedeji, Opeoluwa Oyehan
Aderibigbe, Blessing Atim
Morifi, Eric
Fonkui, Youmbi Thierry
Ndinteh, Derek Tantoh
Nell, Margo Judith
Steenkamp, Vanessa
Journal Title
Journal ISSN
Volume Title
Publisher
Bentham Science Publishers
Abstract
BACKGROUND : Infectious diseases, as well as cancer, are the leading causes of death worldwide. Drug resistance usually results in their treatment requiring a combination of two or more drugs.
OBJECTIVE : Oleanolic-based hybrid compounds were prepared via esterification and characterized using FTIR, NMR and LC-MS. In vitro antibacterial and in vitro cytotoxicity studies were performed.
METHODS : Oleanolic acid was hybridized with selected known pharmaceutical scaffolds via the carboxylic acid functionality in order to develop therapeutics with increased biological activity. Antibacterial activity was determined using the micro-dilution assay against selected Gram-positive and Gram-negative bacteria and cytotoxicity using the sulforhodamine B assay.
RESULTS : Compound 8 displayed potent antibacterial effect against five strains of bacteria, such as Bacillus subtilis, Staphylococcus aureus, Proteus vulgaris, Klebsiella oxytoca, and Escherichia coli, with MIC values of 1.25, 0.078, 0.078, 1.25, 1.25 mg/mL when compared to the control, oleanolic acid (MIC = 2.5 mg/mL). Furthermore, in vitro cytotoxicity, as determined using the SRB assay, against selected cancer cells revealed that compound 7 was the most cytotoxic on MDA, DU145, and MCF-7 cell lines with IC50 values of 69.87 ± 1.04, 73.2 ± 1.08, and 85.27 ± 1.02 μg/mL, respectively, compared to oleanolic acid with an IC50 > 200 μg/mL.
CONCLUSION : Hybridization of oleanolic acid was successful, and further development of these potential antibacterial compounds with reduced cytotoxicity is therefore warranted.
Description
Keywords
4-aminosalicylic acid, Oleanolic acid, Antibacterial, Anticancer, Curcumin, Hybrid compounds
Sustainable Development Goals
Citation
Khwaza, V., Oyedeji, O.O., Aderibigbe, B.A. et al. 2022, 'Design of oleanolic acid-based hybrid compounds as potential pharmaceutical scaffolds', Letters in Drug Design and Discovery, vol. 19, no. 1, pp. 10-19, doi : 10.2174/1570180818666210604112451.