Diagnostic markers for Teratosphaeria destructans and closely related species

Havenga, Minette; Wingfield, Brenda D.; Wingfield, Michael J.; Dreyer, Leanne Laurette; Roets, Francois; Aylward, Janneke

Diagnostic markers for Teratosphaeria destructans and closely related species

dc.contributor.author	Havenga, Minette
dc.contributor.author	Wingfield, Brenda D.
dc.contributor.author	Wingfield, Michael J.
dc.contributor.author	Dreyer, Leanne Laurette
dc.contributor.author	Roets, Francois
dc.contributor.author	Aylward, Janneke
dc.contributor.email	havenga.minette@fabi.up.ac.za	en_ZA
dc.date.accessioned	2021-06-02T10:02:15Z
dc.date.issued	2020-12
dc.description	Supplementary material: Table S1. Teratosphaeria isolates and their sequences used for phylogenetic analyses.	en_ZA
dc.description	Table S2. Microsatellite markers and panel designs for Teratosphaeria species.	en_ZA
dc.description	Table S3. BLASTn results of microsatellites in the Teratosphaeria genomes.	en_ZA
dc.description.abstract	Teratosphaeria foliar pathogens cause leaf and shoot blight on Eucalyptus trees in many parts of the world. Among them, T. destructans is one of the most aggressive pathogens causing defoliation of young Eucalyptus trees in tropic regions. Identification of T. destructans to species level is currently not possible based solely on morphological characteristics or ITS sequence data. The aim of this study was to assess T. destructans microsatellites and a newly developed T. epicoccoides microsatellite as a diagnostic tool to differentiate among T. destructans and several closely related foliar pathogens. Based on the number of markers that amplified, the T. destructans microsatellites allowed for the differentiation of T. destructans, T. epicoccoides,T. eucalypti,T. nubilosa,T. pseudoeucalypti and T. viscidus. These microsatellites provide a rapid and cost-effective diagnostic tool that will enable the identification of a large number of isolates important in disease surveys and inoculation trials.	en_ZA
dc.description.department	Biochemistry	en_ZA
dc.description.department	Forestry and Agricultural Biotechnology Institute (FABI)	en_ZA
dc.description.department	Genetics	en_ZA
dc.description.department	Microbiology and Plant Pathology	en_ZA
dc.description.embargo	2021-10-27
dc.description.librarian	hj2021	en_ZA
dc.description.sponsorship	The National Research Foundation, the SARChI Chair in Fungal Genomics, the UP Doctoral Research Bursary and the Tree Protection Co-operative program (TPCP).	en_ZA
dc.description.uri	http://wileyonlinelibrary.com/journal/efp	en_ZA
dc.identifier.citation	Havenga M, Wingfield BD, Wingfield MJ, Dreyer LL, Roets F, Aylward J. Diagnostic markers for Teratosphaeria destructans and closely related species. Forest Pathology. 2020;50:e12645. https://doi.org/10.1111/efp.12645.	en_ZA
dc.identifier.issn	1437-4781 (print)
dc.identifier.issn	1439-0329 (online)
dc.identifier.other	10.1111/efp.12645
dc.identifier.uri	http://hdl.handle.net/2263/80200
dc.language.iso	en	en_ZA
dc.publisher	Wiley	en_ZA
dc.rights	© 2020 Wiley-VCH GmbH. This is the pre-peer reviewed version of the following article : Diagnostic markers for Teratosphaeria destructans and closely related species. Forest Pathology. 2020;50:e12645. https://doi.org/10.1111/efp.12645. The definite version is available at : http://wileyonlinelibrary.com/journal/efp.	en_ZA
dc.subject	Eucalyptus	en_ZA
dc.subject	Foliar pathogens	en_ZA
dc.subject	Population genetics	en_ZA
dc.subject	Teratosphaeria destructans	en_ZA
dc.subject	Diagnostic markers	en_ZA
dc.title	Diagnostic markers for Teratosphaeria destructans and closely related species	en_ZA
dc.type	Postprint Article	en_ZA