Transient gene expression assays in rose tissues using a Bio-Rad Helios® hand-held gene gun

Kuriakose, Boney; Du Toit, Elsa Sophia; Jordaan, Anton

Transient gene expression assays in rose tissues using a Bio-Rad Helios® hand-held gene gun

dc.contributor.author	Kuriakose, Boney
dc.contributor.author	Du Toit, Elsa Sophia
dc.contributor.author	Jordaan, Anton
dc.contributor.email	boney.kuriakose@up.ac.za	en_US
dc.date.accessioned	2011-09-05T10:14:55Z
dc.date.available	2011-09-05T10:14:55Z
dc.date.issued	2012-01
dc.description.abstract	Rose tissues of different varieties were transformed using a Bio-Rad Helios® hand-held biolistic gun. Parameters for optimum transient expression were optimized and included rose variety, flower age, tissue, gold particle size, DNA loading ratio. Smooth flowers without thick waxy layers and young unopened actively growing flowers were found to be better suited for the transient expression assays. The DNA amounts, gold particle amounts and size etc were not found to influence the efficiency of the transient transformation in these tissues. These studies indicate that biolistic transformation using hand-held guns can be used for successful transient expression assays in rose flower tissues. This is especially useful for a quick and easy analysis of genes and their expression before attempting stable transformation.	en
dc.description.uri	http://www.elsevier.com/locate/sajb	en_US
dc.identifier.citation	Kuriakose, B, Du Toit, ES & Jordaan, A 2012, 'Transient gene expression assays in rose tissues using a Bio-Rad Helios® hand-held gene gun', South African Journal Botany, vol. 78, pp. 307-311, doi:10.1016/j.sajb.2011.06.002.	en
dc.identifier.issn	0254-6299 (print)
dc.identifier.issn	1727-9321 (online)
dc.identifier.other	10.1016/j.sajb.2011.06.002
dc.identifier.uri	http://hdl.handle.net/2263/17219
dc.language.iso	en	en_US
dc.publisher	Elsevier	en_US
dc.rights	© 2011 Elsevier B.V. All rights reserved. Published under a Creative Commons license.	en_US
dc.subject	Biolistic transformation	en
dc.subject	Gene gun	en
dc.subject	Rose flower tissues	en
dc.subject	Rosa hybrida	en
dc.subject	Transient gene expression assays	en
dc.title	Transient gene expression assays in rose tissues using a Bio-Rad Helios® hand-held gene gun	en
dc.type	Article	en