Survey of Citrus tristeza virus (CTV) diversity in pigmented Citrus x paradisi (Macfad.) (Grapefruit) trees in north-western Argentina

dc.contributor.authorRead, David Alan
dc.contributor.authorPalacios, M.F.
dc.contributor.authorKleynhans, Jackie
dc.contributor.authorFigueroa, J.
dc.contributor.authorFoguet, L.
dc.contributor.authorStein, B.
dc.contributor.authorPietersen, Gerhard
dc.contributor.emailgerhard.pietersen@up.ac.zaen_ZA
dc.date.accessioned2017-12-06T11:06:09Z
dc.date.issued2018-06
dc.description.abstractCitrus tristeza virus (CTV) is the most severe viral pathogen of citrus and elicits a wide range of devastating disease symptoms. Grapefruit cultivars (Citrus x paradisi) are the most sensitive among citrus to the effects of CTV infections. Grapefruit is an important crop within the north-western Argentine citrus industry; however, production has been affected by CTV stem-pitting. In general, CTV diversity within South America is poorly studied, with data on grapefruit CTV populations being particularly limited. In this study, 50 samples were collected from Star Ruby, Henninger’s Ruby and Ruben Pink cultivars, within the provinces of Tucumán, Salta and Jujuy in north-western Argentina. The CTV p33 gene was PCR amplified and the resulting amplicons sequenced with Sanger sequencing. A subset of these amplicons was sequenced with Illumina MiSeq sequencing. AT-1-like sequences were dominant within the majority of populations, as determined by Sanger sequencing, followed by sequences clustering within the unresolved Kpg3/SP/T3 and RB clades. Sequencing by Illumina MiSeq confirmed this, as well as detecting minor sequence types within the HA 16–5, VT, B165 and A18 clades.en_ZA
dc.description.departmentMicrobiology and Plant Pathologyen_ZA
dc.description.embargo2019-06-01
dc.description.librarianhj2017en_ZA
dc.description.sponsorshipThe National Research Foundation (South Africa) and Ministerio de Ciencia, Tecnologia e Innovación Productiva (Argentina) via the South Africa-Argentina Bilateral agreement programme.en_ZA
dc.description.urihttp://link.springer.com/journal/10658en_ZA
dc.identifier.citationRead, D.A., Palacios, M.F., Kleynhans, J. et al. Survey of Citrus tristeza virus (CTV) diversity in pigmented Citrus x paradisi (Macfad.) (Grapefruit) trees in north-western Argentina. European Journal of Plant Pathology (2018) 151: 329-340. https://doi.org/10.1007/s10658-017-1376-5.en_ZA
dc.identifier.issn0929-1873 (print)
dc.identifier.issn1573-8469 (online)
dc.identifier.other10.1007/s10658-017-1376-5
dc.identifier.urihttp://hdl.handle.net/2263/63448
dc.language.isoenen_ZA
dc.publisherSpringeren_ZA
dc.rights© Koninklijke Nederlandse Planteziektenkundige Vereniging 2017. The original publication is available at : http://link.springer.comjournal/10658.en_ZA
dc.subjectCitrus tristeza virus (CTV)en_ZA
dc.subjectGrapefruiten_ZA
dc.subjectArgentinaen_ZA
dc.subjectIllumina sequencingen_ZA
dc.titleSurvey of Citrus tristeza virus (CTV) diversity in pigmented Citrus x paradisi (Macfad.) (Grapefruit) trees in north-western Argentinaen_ZA
dc.typePostprint Articleen_ZA

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