An evaluation of the bacterial diversity at Tshipise, Mphephu and Sagole hot water springs, Limpopo Province, South Africa

dc.contributor.authorTekere, Memory
dc.contributor.authorPrinsloo, Adele
dc.contributor.authorOlivier, J. (Jana), 1948-
dc.contributor.authorJonker, Nelia
dc.contributor.authorVenter, S.N. (Stephanus Nicolaas)
dc.date.accessioned2012-10-16T07:11:03Z
dc.date.available2012-10-16T07:11:03Z
dc.date.issued2012-06-21
dc.description.abstractTshipise, Mphephu and Sagole are thermal hot water springs in the Limpopo Province of South Africa with temperatures of 58, 43 and 45°C; and pH of 8.85, 8.08 and 9.70, respectively. The bacterial diversity of the hot water springs was determined by pyrosequencing of the two 16S rRNA hypervariable regions V1-3 and V4-7. Analyses of the community DNA revealed that bacterial populations as detectable by the V1-3 or V4-7 region, respectively were dominated by the Bacteriodetes and Proteobacteria for Mphephu, and Proteobacteria and Cyanobacteria for both Tshipise and Sagole. The major differences in the bacterial diversity between the springs was that no Cyanobacteria were detected for Mphephu and the level of Bacteriodetes detected for both Tshipise and Sagole was much lower compared to the levels detected at Mphephu. The Firmicutes were detected at all the springs but at a much lower abundance compared to the other main phyla detected. Various other phyla were detected at the hot springs at levels below 0.20% of the total sequences obtained. It is interesting that very diverse bacterial genera exist in the three hot water springs studied.en_US
dc.description.sponsorshipThis research was supported through a grant from Water Research Commission (WRC, SA, Project K5/1959/1).en_US
dc.description.urihttp://www.academicjournals.org/AJMRen_US
dc.identifier.citationTekere, M, Prinsloo, A, Olivier, J, Jonker, N & Venter, S 2012, 'An evaluation of the bacterial diversity at Tshipise, Mphephu and Sagole hot water springs, Limpopo Province, South Africa', African Journal of Microbiology Research, vol. 6, no. 23, pp. 4993-5004.en_US
dc.identifier.issn1996-0808
dc.identifier.other10.5897/AJMR12.250
dc.identifier.urihttp://hdl.handle.net/2263/20176
dc.language.isoenen_US
dc.publisherAcademic Journalsen_US
dc.rights© 2012 Academic Journalsen_US
dc.subjectHot springsen_US
dc.subjectBacterial diversityen_US
dc.subjectMetagenomeen_US
dc.subjectSouth Africaen_US
dc.titleAn evaluation of the bacterial diversity at Tshipise, Mphephu and Sagole hot water springs, Limpopo Province, South Africaen_US
dc.typeArticleen_US

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