Comparative transcriptional analysis of Persea americana MYB, WRKY and AP2/ERF transcription factors following Phytophthora cinnamomi infection

dc.contributor.authorFick, Alicia
dc.contributor.authorSwart, Velushka
dc.contributor.authorBombarely, Aureliano
dc.contributor.authorVan den Berg, Noelani
dc.contributor.emailnoelani.vandenberg@up.ac.zaen_US
dc.date.accessioned2024-05-10T06:58:11Z
dc.date.available2024-05-10T06:58:11Z
dc.date.issued2024-04
dc.descriptionDATA AVAILABILITY STATEMENT : The data sets used in this study can be found in online repositories. Raw RNA-seq data used in this study has been deposited in the Sequence Read Archive of NCBI GenBank at https://www.ncbi.nlm.nih.gov/sra under accession number PRJNA675400.en_US
dc.description.abstractPlant cells undergo extensive transcriptional reprogramming following pathogen infection, with these reprogramming patterns becoming more complex when pathogens, such as hemibiotrophs, exhibit different lifestyles. These transcriptional changes are often orchestrated by MYB, WRKY and AP2/ERF transcription factors (TFs), which modulate both growth and defence-related gene expression. Transcriptional analysis of defence-related genes in avocado (Persea americana) infected with Phytophthora cinnamomi indicated differential immune response activation when comparing a partially resistant and susceptible rootstock. This study identified 226 MYB, 82 WRKY, and 174 AP2/ERF TF-encoding genes in avocado, using a genome-wide approach. Phylogenetic analysis revealed substantial sequence conservation within TF groups underscoring their functional significance. RNA-sequencing analysis in a partially resistant and susceptible avocado rootstock infected with P. cinnamomi was indicative of an immune response switch occurring in either rootstock after 24 and 6 h post-inoculation, respectively. Different clusters of co-expressed TF genes were observed at these times, suggesting the activation of necrotroph-related immune responses at varying intervals between the two rootstocks. This study aids our understanding of avocado immune response activation following P. cinnamomi infection, and the role of the TFs therein, elucidating the transcriptional reprogramming disparities between partially resistant and susceptible rootstocks.en_US
dc.description.departmentBiochemistryen_US
dc.description.departmentForestry and Agricultural Biotechnology Institute (FABI)en_US
dc.description.departmentGeneticsen_US
dc.description.departmentMicrobiology and Plant Pathologyen_US
dc.description.librarianhj2024en_US
dc.description.sdgSDG-15:Life on landen_US
dc.description.sponsorshipHans Merensky Foundationen_US
dc.description.urihttp://www.wileyonlinelibrary.com/journal/mppen_US
dc.identifier.citationFick, A., Swart, V., Bombarely, A. & van den Berg, N. (2024) Comparative transcriptional analysis of Persea americana MYB, WRKY and AP2/ERF transcription factors following Phytophthora cinnamomi infection. Molecular Plant Pathology, 25, e13453. Available from: https://doi.org/10.1111/mpp.13453.en_US
dc.identifier.issn1464-6722 (print)
dc.identifier.issn1364-3703 (online)
dc.identifier.other10.1111/mpp.13453
dc.identifier.urihttp://hdl.handle.net/2263/95873
dc.language.isoenen_US
dc.publisherWileyen_US
dc.rights© 2024 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License.en_US
dc.subjectAvocado (Persea americana)en_US
dc.subjectDefence-related genesen_US
dc.subjectEthylene-responsive factor (ERF)en_US
dc.subjectMYBen_US
dc.subjectPhytophthoraen_US
dc.subjectTranscription factorsen_US
dc.subjectWRKYen_US
dc.subjectSDG-15: Life on landen_US
dc.titleComparative transcriptional analysis of Persea americana MYB, WRKY and AP2/ERF transcription factors following Phytophthora cinnamomi infectionen_US
dc.typeArticleen_US

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