In vitro cultivation of Babesia equi: detection of carrier animals and isolation of parasites

dc.contributor.authorJust, M.C.
dc.contributor.authorDe Waal, D.T.
dc.contributor.editorVerwoerd, Daniel Wynand
dc.contributor.upauthorZweygarth, Erich
dc.date.accessioned2012-12-12T07:11:45Z
dc.date.available2012-12-12T07:11:45Z
dc.date.created2012
dc.date.issued1997
dc.descriptionThe articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.en_US
dc.description.abstractBy means of an in vitro culture technique, 75 samples of horse blood were examined for Babesia equi, a causative agent of equine piroplasmosis. At the time of culture initiation, 15 samples were microscopically positive for B. equi, and this was subsequently confirmed by culture diagnosis. Sixty samples showed no parasites in Giemsa-stained thin blood smears. However, after the culturing process, parasites were found in blood smears of 36 of these samples. The sensitivity of the in vitro culture method was such that 2,5 µl (1/40 of the usual volume used for the above mentioned samples} of packed erythrocytes obtained from a carrier horse still yielded positive results after cultivation. Cultures were initiated from blood samples stored for up to 120 h at 8º C in vacuum tubes containing EDTA as anticoagulant. These results show that the in vitro culture method is highly sensitive. It can be used to identify B. equi carrier horses, to evaluate the effects of chemotherapeutic intervention, and to isolate field strains of B. equi or further characterization.en_US
dc.identifier.citationZweygarth, E, Just, MC & De Waal, DT 1997, 'In vitro cultivation of Babesia equi: detection of carrier animals and isolation of parasites’. Onderstepoort Journal of Veterinary Research, vol. 64, no. 1, pp. 51-56.en_US
dc.identifier.issn0330-2465
dc.identifier.urihttp://hdl.handle.net/2263/20708
dc.language.isoenen_US
dc.publisherPublished by the Agricultural Research Council, Onderstepoort Veterinary Instituteen_US
dc.rights© ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital).en_US
dc.subjectVeterinary medicineen_US
dc.subjectBabesia equien_US
dc.subjectIn vitro cultivationen_US
dc.subjectCarrier animalsen_US
dc.subjectParasite isolationen_US
dc.subject.lcshVeterinary medicine -- South Africa
dc.titleIn vitro cultivation of Babesia equi: detection of carrier animals and isolation of parasitesen_US
dc.typeArticleen_US

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