Antibiogram signatures of some enterobacteria recovered from irrigation water and agricultural soil in two district municipalities of South Africa

dc.contributor.authorIwu, Chidozie Declan
dc.contributor.authorDu Plessis, Erika Margarete
dc.contributor.authorKorsten, Lise
dc.contributor.authorNontongana, Nolonwabo
dc.contributor.authorOkoh, Anthony Ifeanyi
dc.contributor.emailerika.duplessis@up.ac.zaen_ZA
dc.date.accessioned2021-05-21T11:37:56Z
dc.date.available2021-05-21T11:37:56Z
dc.date.issued2020-08-07
dc.descriptionTable S1: Description of sampling points, Table S2: Primer sequence and PCR cycling conditions used for the molecular detection of members of Enterobacteriales, Table S3: The primer sequence and expected amplicon size used for the screening of resistance genes in members of Enterobacteriales, Table S4: The primer sequence and expected amplicon size used for the screening of AmpC -lactamase and ESBLs in members of Enterobacteriales [44].en_ZA
dc.description.abstractThis study was undertaken to evaluate the antibiogram fingerprints of some Enterobacteria recovered from irrigation water and agricultural soil in two District Municipalities of the Eastern Cape Province, South Africa using standard culture-based and molecular methods. The prevalent resistance patterns in the isolates follow the order: Salmonella enterica serovar Typhimurium [tetracycline (92.3%), ampicillin (69.2%)]; Enterobacter cloacae [amoxicillin/clavulanic acid (77.6%), ampicillin (84.5%), cefuroxime (81.0%), nitrofurantoin (81%), and tetracycline (80.3%)]; Klebsiella pneumoniae [amoxicillin/clavulanic acid (80.6%), ampicillin (88.9%), and cefuroxime (61.1%)]; and Klebsiella oxytoca [chloramphenicol (52.4%), amoxicillin/clavulanic acid (61.9%), ampicillin (61.9%), and nitrofurantoin (61.9%)]. Antibiotic resistance genes detected include tetC (86%), sulII (86%), and blaAmpC (29%) in Salmonella enterica serovar Typhimurium., tetA (23%), tetB (23%), tetC (12%), sulI (54%), sulII (54%), catII (71%), blaAmpC (86%), blaTEM (43%), and blaPER (17%) in Enterobacter cloacae., tetA(20%), tetC (20%), tetD(10%), sulI (9%), sulII (18%), FOX (11%) and CIT (11%)-type plasmid-mediated AmpC, blaTEM (11%), and blaSHV (5%) in Klebsiella pneumoniae and blaAmpC (18%) in Klebsiella oxytoca. Our findings document the occurrence of some antibiotic-resistant Enterobacteria in irrigation water and agricultural soil in Amathole and Chris Hani District Municipalities, Eastern Cape Province of South Africa, thus serving as a potential threat to food safety.en_ZA
dc.description.departmentPlant Production and Soil Scienceen_ZA
dc.description.librarianam2021en_ZA
dc.description.sponsorshipThe South African Medical Research Council (SAMRC), United States Agency for International Development (USAID) and the National Research Foundation (NRF).en_ZA
dc.description.urihttp://www.mdpi.com/journal/microorganismsen_ZA
dc.identifier.citationIwu, C.D., Du Plessis, E.M., Korsten, L. et al. 2020, 'Antibiogram signatures of some enterobacteria recovered from irrigation water and agricultural soil in two district municipalities of South Africa', Microorganisms, vol. 8, art. 1206, pp. 1-19.en_ZA
dc.identifier.issn2076-2607 (online)
dc.identifier.other10.3390/microorganisms8081206
dc.identifier.urihttp://hdl.handle.net/2263/80000
dc.language.isoenen_ZA
dc.publisherMDPIen_ZA
dc.rights© 2020 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en_ZA
dc.subjectAntibiotic resistanceen_ZA
dc.subjectIrrigation wateren_ZA
dc.subjectAgricultural soilen_ZA
dc.subjectPublic healthen_ZA
dc.subjectFood safetyen_ZA
dc.subjectFood microbiologyen_ZA
dc.subjectEnvironmental healthen_ZA
dc.subjectSouth Africa (SA)en_ZA
dc.titleAntibiogram signatures of some enterobacteria recovered from irrigation water and agricultural soil in two district municipalities of South Africaen_ZA
dc.typeArticleen_ZA

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