UPLC-MS analysis of cannabis sativa using tetrahydrocannabinol (THC), cannabidiol (CBD), and tetrahydrocannabinolic acid (THCA) as marker compounds: inhibition of breast cancer cell survival and progression
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Date
Authors
Bala, Asis
Rademan, Sunelle
Kevin, Khorommbi Ndivhuwo
Maharaj, Vinesh J.
Matsabisa, Motlalepula G.
Journal Title
Journal ISSN
Volume Title
Publisher
Sage
Abstract
Cannabis sativa L. extracts were characterized by ultra performance liquid chromatography-mass spectrometry (UPLC-MS) using
tetrahydrocannabinol (THC), cannabidiol (CBD), and tetrahydrocannabinolic acid (THCA) as marker compounds. The inhibitory
effects of various extracts were determined on the survival and progression of highly metastatic breast cancer cells. A higher
amount of CBD was found in the dichloromethane extract, and this was found to be effective in inhibiting breast cancer cell
growth in vitro and in angiogenesis. Collectively, it may be concluded that CBD, THC, and THCA in the African variety of C. sativa
can be used as marker compounds in UPLC-MS analysis. The ability of the plant to inhibit breast cancer cell survival and progression
may affirm the traditional use of the drug as an anticancer agent.
Description
Keywords
Breast cancer, Angiogenesis, Cannabis sativa L. extracts, Ultra performance liquid chromatography-mass spectrometry (UPLC-MS), Tetrahydrocannabinol (THC), Tetrahydrocannabinolic acid (THCA), Cannabidiol (CBD)
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Citation
Bala A, Rademan S, Kevin KN, Maharaj V, Matsabisa MG. UPLC-MS Analysis of Cannabis sativa Using Tetrahydrocannabinol (THC), Cannabidiol (CBD), and Tetrahydrocannabinolic Acid (THCA) as Marker Compounds: Inhibition of Breast Cancer Cell Survival and Progression. Natural Product Communications. 14(8):1-5, 2019. doi:10.1177/1934578X19872907.