Population genetic structure of the parasitic nematode Spirocerca lupi in South Africa

dc.contributor.authorGreeff, Jacobus Maree
dc.contributor.authorReid, Kerry
dc.contributor.authorGagjee, Janishtha R.
dc.contributor.authorClift, Sarah Jane
dc.contributor.authorDe Waal, Pamela Jean
dc.contributor.emailjaco.greeff@up.ac.zaen_ZA
dc.date.accessioned2018-08-28T10:01:07Z
dc.date.issued2018-07
dc.descriptionSupplementary data S1: Excel file of data.en_ZA
dc.descriptionSupplementary text: The use and meaning of F-statistics.en_ZA
dc.description.abstractSpirocerca lupi is a parasitic nematode of canids and occurs in most tropical and subtropical regions around the world. While its life cycle is well known, insight is lacking about its mating structure within-hosts, genetic variability and long-distance dispersal ability. These characteristics contribute significantly to the dynamics and spread of potential resistance genes, which impacts on the control of S. lupi. To evaluate the population structure and infer potential mating behaviour of S. lupi, we genotyped 130 samples at nine microsatellite loci from three geographical locations in South Africa, between 600 and 1000 km apart. These loci identified unique individuals with high levels of polymorphism suggesting that these are not newly established S. lupi populations in South Africa and that effective population sizes must be large. Population genetic analyses showed that populations are not very distinct, that worms within dogs are more similar to each other than random worms from each population, and that mating is at random within dogs. We can thus infer that the parasite is frequently transported over great distances. Even so, two genetically distinct populations could be identified. Relatedness of worms within dogs were significantly higher than between dogs and together with F-statistics suggests some non-random transmission of parasites between hosts. While mating is random within a host, parasites from a host are more likely to be related and hence an increase in homozygosity is seen. The implications of this genetic structure on parasite control are considered.en_ZA
dc.description.departmentBiochemistryen_ZA
dc.description.departmentGeneticsen_ZA
dc.description.departmentMicrobiology and Plant Pathologyen_ZA
dc.description.departmentParaclinical Sciencesen_ZA
dc.description.embargo2019-07-15
dc.description.librarianhj2018en_ZA
dc.description.sponsorshipThe Animal and Zoonotic Diseases Institutional Research Theme at the University of Pretoria and in part by a number of grants from the National Research Foundation of South Africa (UID: 78566 (NRF RISP grant for the ABI3500) and grant number 80678 to PdW and 77256 to JMG).en_ZA
dc.description.urihttp://www.elsevier.com/locate/vetparen_ZA
dc.identifier.citationGreeff, J.M., Reid, K., Gagjee, J.R. et al. 2018, 'Population genetic structure of the parasitic nematode Spirocerca lupi in South Africa', Veterinary Parasitology, vol. 258, pp. 64-69.en_ZA
dc.identifier.issn0304-4017 (print)
dc.identifier.issn1873-2550 (online)
dc.identifier.other10.1016/j.vetpar.2018.06.007
dc.identifier.urihttp://hdl.handle.net/2263/66346
dc.language.isoenen_ZA
dc.publisherElsevieren_ZA
dc.rights© 2018 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Veterinary Parasitology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Veterinary Parasitology, vol. 258, pp. 64-69, 2018. doi : 10.1016/j.vetpar.2018.06.007.en_ZA
dc.subjectCanidsen_ZA
dc.subjectGene flowen_ZA
dc.subjectMicrosatellitesen_ZA
dc.subjectF-statisticsen_ZA
dc.subjectPopulation structureen_ZA
dc.subjectRelatednessen_ZA
dc.subjectSouth Africa (SA)en_ZA
dc.subjectSpirocerca lupi nematodesen_ZA
dc.subjectSpirocerca lupi wormsen_ZA
dc.subjectWormsen_ZA
dc.subjectAnimal experimenten_ZA
dc.subjectPopulation genetic structureen_ZA
dc.subjectParasite controlen_ZA
dc.subjectMicrosatellite markersen_ZA
dc.subjectMatingen_ZA
dc.subjectHomozygosityen_ZA
dc.subjectGenetic associationen_ZA
dc.subjectGenetic analysisen_ZA
dc.subjectGene mutationen_ZA
dc.titlePopulation genetic structure of the parasitic nematode Spirocerca lupi in South Africaen_ZA
dc.typePostprint Articleen_ZA

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