New isolations of the rabies-related Mokola virus from South Africa

Coertse, Jessica; Markotter, Wanda; Le Roux, Kevin; Stewart, Daniel; Sabeta, Claude Taurai; Nel, Louis Hendrik

New isolations of the rabies-related Mokola virus from South Africa

dc.contributor.author	Coertse, Jessica
dc.contributor.author	Markotter, Wanda
dc.contributor.author	Le Roux, Kevin
dc.contributor.author	Stewart, Daniel
dc.contributor.author	Sabeta, Claude Taurai
dc.contributor.author	Nel, Louis Hendrik
dc.contributor.email	louis.nel@up.ac.za	en_ZA
dc.date.accessioned	2017-03-29T05:55:55Z
dc.date.available	2017-03-29T05:55:55Z
dc.date.issued	2017-01-31
dc.description	Additional file 1: Table S1. Primers and PCR conditions for amplification of the Nucleoprotein-, Phosphoprotein-, Matrix protein- and Glycoprotein genes of Mokola virus isolates.	en_ZA
dc.description	Additional file 2: Table S2. Details of sequences used for the Bayesian analysis of the rabies virus positive samples.	en_ZA
dc.description	Additional file 3: Table S3. Details of sequences used for the Bayesian analysis of the new Mokola virus isolates.	en_ZA
dc.description	Additional file 4: Figure S1. Bayesian analysis of the coding region of the Nucleoprotein gene (1353 bp) of all Mokola virus isolates (Additional file 3: Table S3) applying the general time reversible substitution model with invariable sites. Laboratory reference numbers are shown for all sequences, followed by the host species, country of origin (KZN SA: KwaZulu-Natal province, South Africa; EC SA: Eastern Cape province South Africa; ZIM: Zimbabwe; CAR: Central African Republic; NIG: Nigeria) and year of isolation.	en_ZA
dc.description	Additional file 5: Figure S2. Bayesian analysis of the coding region of the Phosphoprotein gene (913 bp) applying the general time reversible substitution model with gamma distribution. Laboratory reference numbers are shown for all sequences, followed by the host species, country of origin (KZN SA: KwaZulu-Natal province, South Africa; EC SA: Eastern Cape province South Africa; ZIM: Zimbabwe; CAR: Central African Republic; NIG: Nigeria) and year of isolation.	en_ZA
dc.description	Additional file 6: Figure S3. Bayesian analysis of the coding region of the Matrix protein gene (609 bp) applying the general time reversible substitution model with gamma distribution. Laboratory reference numbers are shown for all sequences, followed by the host species, country of origin (KZN SA: KwaZulu-Natal province, South Africa; EC SA: Eastern Cape province South Africa; ZIM: Zimbabwe; CAR: Central African Republic; NIG: Nigeria) and year of isolation.	en_ZA
dc.description	Additional file 7: Figure S4. Bayesian analysis of the coding region of the Glycoprotein gene (1569 bp) applying the general time reversible substitution model with gamma distribution and invariable sites. Laboratory reference numbers are shown for all sequences, followed by the host species, country of origin (KZN SA: KwaZulu-Natal province, South Africa; EC SA: Eastern Cape province South Africa; ZIM: Zimbabwe; CAR: Central African Republic; NIG: Nigeria) and year of isolation.	en_ZA
dc.description	Additional file 8: Table S4. Nucleotide identity of the Nucleoprotein gene of all Mokola virus isolates.	en_ZA
dc.description	Additional file 9: Table S5. Nucleotide identity of the Phosphoprotein gene of all Mokola virus isolates.	en_ZA
dc.description	Additional file 10: Table S6. Nucleotide identity of the Matrix protein gene of all Mokola virus isolates.	en_ZA
dc.description	Additional file 11: Table S7. Nucleotide identity of the Glycoprotein gene of all Mokola virus isolates.	en_ZA
dc.description.abstract	BACKGROUND : Mokola virus (MOKV) is a rabies-related lyssavirus and appears to be exclusive to the African continent. Only 24 cases of MOKV, which includes two human cases, have been reported since its identification in 1968. MOKV has an unknown reservoir host and current commercial vaccines do not confer protection against MOKV. RESULTS : We describe three new isolations of MOKV from domestic cats in South Africa. Two cases were retrospectively identified from 2012 and an additional one in 2014. CONCLUSIONS : These cases emphasize the generally poor surveillance for rabies-related lyssaviruses and our inadequate comprehension of the epidemiology and ecology of Mokola lyssavirus per se.	en_ZA
dc.description.department	Medical Virology	en_ZA
dc.description.department	Microbiology and Plant Pathology	en_ZA
dc.description.librarian	am2017	en_ZA
dc.description.sponsorship	This work was partially funded by the National Research Foundation (Grant UID 92524 & RISP grant UID78566), the Poliomyelitis Research Foundation (Grant no. 10/40, 12/14) and the Animal and Zoonotic Diseases Institutional Research Theme of the University of Pretoria.	en_ZA
dc.description.uri	http://www.biomedcentral.com/bmcvetres	en_ZA
dc.identifier.citation	Coertse, J, Markotter, W, le Roux, K, Stewart, D, Sabeta, CT & Nel, LH 2017, 'New isolations of the rabies-related Mokola virus from South Africa', BMC Veterinary Research, vol. 13, art. no. 37, pp. 1-8.	en_ZA
dc.identifier.issn	1746-6148
dc.identifier.other	10.1186/s12917-017-0948-0
dc.identifier.uri	http://hdl.handle.net/2263/59559
dc.language.iso	en	en_ZA
dc.publisher	BioMed Central	en_ZA
dc.rights	© The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License.	en_ZA
dc.subject	Lyssavirus	en_ZA
dc.subject	Rabies-related	en_ZA
dc.subject	South Africa (SA)	en_ZA
dc.subject	Mokola virus (MOKV)	en_ZA
dc.title	New isolations of the rabies-related Mokola virus from South Africa	en_ZA
dc.type	Article	en_ZA