Viability reagent, prestoblue, in comparison with other available reagents, utilized in cytotoxicity and antimicrobial assays

dc.contributor.authorLall, Namrita
dc.contributor.authorHenley-Smith, Cynthia Joan
dc.contributor.authorDe Canha, Marco Nuno
dc.contributor.authorOosthuizen, Carel Basson
dc.contributor.authorBerrington, Danielle
dc.date.accessioned2014-05-20T13:10:38Z
dc.date.available2014-05-20T13:10:38Z
dc.date.issued2013
dc.description.abstractThis study compared different commercially available viability reagents. The growth indicator reagents include p-iodonitrotetrazolium violet (INT), PrestoBlue, and Alamar Blue which were used for antimicrobial analysis against Streptococcus mutans, Prevotella intermedia, Propionibacterium acnes, and Mycobacterium tuberculosis. PrestoBlue and Alamar Blue are resazurin based reagents that resulted in a quick and easily distinguishable colour change that allowed for visual readings. INT and Sodium 3′-[1-(phenyl amino-carbonyl)-3,4-tetrazolium]-bis-[4-methoxy-6-nitro] benzene sulfonic acid hydrate (XTT) are tetrazolium based reagents which are converted to a formazan dye in the presence of metabolically active mitochondria enzyme. For cell viability analysis, reagents XTT and PrestoBlue were compared. PrestoBlue was able to clearly indicate the minimum inhibitory concentration (MIC) of various positive drug controls on various microbial strains. PrestoBlue was also a good indicator of the 50% inhibitory concentration (IC50) of positive drug controls on various cell lines.en_US
dc.description.librarianhb2014en_US
dc.description.urihttp://www.hindawi.comen_US
dc.identifier.citationLall, N, Henley-Smith, CJ, De Canha, MN, Oosthuizen, CB & Berrington, D 2013, 'Viability reagent, prestoblue, in comparison with other available reagents, utilized in cytotoxicity and antimicrobial assays', International Journal of Microbiology, vol. 2013, art. 420601, pp. 1-5.en_US
dc.identifier.issn1687-918X (print)
dc.identifier.issn1687-9198 (online)
dc.identifier.other10.1155/2013/420601
dc.identifier.urihttp://hdl.handle.net/2263/39824
dc.language.isoenen_US
dc.publisherHindawi Publishing Corporationen_US
dc.rights© 2013 Namrita Lall et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.subjectViability reagenten_US
dc.subjectPrestoblueen_US
dc.subjectComparisonen_US
dc.subjectAvailable reagentsen_US
dc.subjectUtilizeden_US
dc.subjectCytotoxicityen_US
dc.subjectAntimicrobial assaysen_US
dc.titleViability reagent, prestoblue, in comparison with other available reagents, utilized in cytotoxicity and antimicrobial assaysen_US
dc.typeArticleen_US

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