Screening of candidate bioactive secondary plant metabolite Ion-features from Moringa oleifera accessions associated with high and low enteric methane inhibition from ruminants

dc.contributor.authorZeru, Addisu Endalew
dc.contributor.authorHassen, Abubeker
dc.contributor.authorApostolides, Zeno
dc.contributor.authorTjelele, Julius
dc.contributor.emailabubeker.hassen@up.ac.zaen_US
dc.date.accessioned2023-09-18T08:23:35Z
dc.date.available2023-09-18T08:23:35Z
dc.date.issued2022-05-31
dc.descriptionDATA AVAILABILITY STATEMENT : Data will be stored in the University of Pretoria repository, and access to the data will be granted by making a reasonable request to the University of Pretoria or the corresponding author.en_US
dc.description.abstractThis study evaluated the relationship of secondary bioactive plant metabolite ion-features (MIFs) of Moringa oleifera accessions with antimethanogenesis to identify potential MIFs that were responsible for high and low methane inhibition from ruminants. Plant extracts from 12 Moringa accessions were evaluated at a 50 mg/kg DM feed for gas production and methane inhibition. Subsequently, the accessions were classified into low and high enteric methane inhibition groups. Four of twelve accessions (two the lowest and two the highest methane inhibitors), were used to characterize them in terms of MIFs. A total of 24 samples (12 from lower and 12 from higher methane inhibitors) were selected according to their methane inhibition potential, which ranged from 18% to 29%. Ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) and untargeted metabolomics with univariate and multivariate statistical analysis with MetaboAnalyst were used in the study. Although 86 MIFs showed (p < 0.05) variation between higher and lower methane inhibition groups and lay within the detection ranges of the UPLC-MS column, only 14 were significant with the volcano plot. However, Bonferroni correction reduced the candidate MIFs to 10, and their R2-value with methane production ranged from 0.39 to 0.64. Eventually, MIFs 4.44_609.1462 and MIF 4.53_433.1112 were identified as bioactive MIFs associated with higher methane inhibition, whereas MIF 9.06_443.2317 and 15.00_487.2319 were associated with lower methane inhibition with no significant effect on in vitro organic matter digestibility of the feed. These MIFs could be used by plant breeders as potential markers to develop new M. oleifera varieties with high methane inhibition characteristics. However, further investigation on identifying the name, structure, and detailed biological activities of these bioactive metabolites needs to be carried out for future standardization, commercialization, and application as dietary methane mitigation additives.en_US
dc.description.departmentAnimal and Wildlife Sciencesen_US
dc.description.departmentBiochemistryen_US
dc.description.departmentGeneticsen_US
dc.description.departmentMicrobiology and Plant Pathologyen_US
dc.description.librarianam2023en_US
dc.description.sponsorshipThe National Research Foundation (NRF) of South Africa.en_US
dc.description.urihttps://www.mdpi.com/journal/metabolitesen_US
dc.identifier.citationZeru, A.E.; Hassen, A.; Apostolides, Z.; Tjelele, J. Screening of Candidate Bioactive Secondary Plant Metabolite Ion-Features from Moringa oleifera Accessions Associated with High and Low Enteric Methane Inhibition from Ruminants. Metabolites 2022, 12, 501. https://DOI.org/10.3390/metabo12060501.en_US
dc.identifier.issn2218-1989
dc.identifier.other10.3390/metabo12060501
dc.identifier.urihttp://hdl.handle.net/2263/92303
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rights© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en_US
dc.subjectAccessionen_US
dc.subjectMethane inhibitionen_US
dc.subjectSecondary plant metabolitesen_US
dc.subjectm/z Ion-featuresen_US
dc.subjectRelationshipen_US
dc.subjectRuminantsen_US
dc.subjectMetabolite ion-features (MIFs)en_US
dc.titleScreening of candidate bioactive secondary plant metabolite Ion-features from Moringa oleifera accessions associated with high and low enteric methane inhibition from ruminantsen_US
dc.typeArticleen_US

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