Whole genome sequence analysis of Brucella abortus isolates from various regions of South Africa

dc.contributor.authorLedwaba, Maphuti Betty
dc.contributor.authorGlover, Barbara
dc.contributor.authorMatle, Itumeleng
dc.contributor.authorProfiti, Giuseppe
dc.contributor.authorMartelli, Pier Luigi
dc.contributor.authorCasadio, Rita
dc.contributor.authorZilli, Katiuscia
dc.contributor.authorJanowicz, Anna
dc.contributor.authorMarotta, Francesca
dc.contributor.authorGarofolo, Giuliano
dc.contributor.authorVan Heerden, Henriette
dc.date.accessioned2022-05-24T08:30:02Z
dc.date.available2022-05-24T08:30:02Z
dc.date.issued2021-03-11
dc.descriptionSUPPLEMENTARY TABLES: Table S1: Sample order used in Bruce-ladder (A) and AMOS (B) multiplex PCR assays and the descriptive information of the gel images, Table S2: Clean unique variants of the South African strains (refer to Table 2 for the sample names (in column 1) and sample ID (in column 2)).en_US
dc.description.abstractThe availability of whole genome sequences in public databases permits genome-wide comparative studies of various bacterial species. Whole genome sequence-single nucleotide polymorphisms (WGS-SNP) analysis has been used in recent studies and allows the discrimination of various Brucella species and strains. In the present study, 13 Brucella spp. strains from cattle of various locations in provinces of South Africa were typed and discriminated. WGS-SNP analysis indicated a maximum pairwise distance ranging from 4 to 77 single nucleotide polymorphisms (SNPs) between the South African Brucella abortus virulent field strains. Moreover, it was shown that the South African B. abortus strains grouped closely to B. abortus strains from Mozambique and Zimbabwe, as well as other Eurasian countries, such as Portugal and India. WGS-SNP analysis of South African B. abortus strains demonstrated that the same genotype circulated in one farm (Farm 1), whereas another farm (Farm 2) in the same province had two different genotypes. This indicated that brucellosis in South Africa spreads within the herd on some farms, whereas the introduction of infected animals is the mode of transmission on other farms. Three B. abortus vaccine S19 strains isolated from tissue and aborted material were identical, even though they originated from different herds and regions of South Africa. This might be due to the incorrect vaccination of animals older than the recommended age of 4–8 months or might be a problem associated with vaccine production.en_US
dc.description.departmentVeterinary Tropical Diseasesen_US
dc.description.librarianam2022en_US
dc.description.sponsorshipThe Gauteng Department of Agriculture and Rural Development (GDARD), National Research Foundation, South Africa and Institute of Tropical Medicine, Belgium.en_US
dc.description.urihttps://www.mdpi.com/journal/microorganismsen_US
dc.identifier.citationLedwaba, M.B.; Glover, B.A.; Matle, I.; Profiti, G.; Martelli, P.L.; Casadio, R.; Zilli, K.; Janowicz, A.; Marotta, F.; Garofolo, G.; et al. Whole Genome Sequence Analysis of Brucella abortus Isolates from Various Regions of South Africa. Microorganisms 2021, 9, 570. https://DOI.org/ 10.3390/microorganisms9030570.en_US
dc.identifier.issn2072-666X (online)
dc.identifier.other10.3390/microorganisms9030570
dc.identifier.urihttps://repository.up.ac.za/handle/2263/85639
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rights© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en_US
dc.subjectBovine brucellosisen_US
dc.subjectBrucella abortusen_US
dc.subjectWhole genome sequencing (WGS)en_US
dc.subjectSingle nucleotide polymorphismsen_US
dc.subjectComparative analysisen_US
dc.titleWhole genome sequence analysis of Brucella abortus isolates from various regions of South Africaen_US
dc.typeArticleen_US

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