The impact of collection tube fill volume on international normalized ratio

dc.contributor.authorPotgieter, Johan
dc.contributor.authorPool, Roger
dc.contributor.authorPrinsloo, Andrea
dc.contributor.authorBotha, Elsabie M.D.
dc.contributor.authorOlorunju, Steve A.S.
dc.contributor.emailjohancp@up.ac.zaen_US
dc.date.accessioned2011-09-06T07:03:39Z
dc.date.available2011-09-06T07:03:39Z
dc.date.issued2010-06
dc.description.abstractINTRODUCTION: Pre-analytical variability currently represents the most important source of errors that can lead to inaccurate patient results in monitoring of patients being treated with oral anticoagulant therapy. The volume of blood collected is critical for accurate coagulation results. The National Committee for Clinical Laboratory Standards (NCCLS) recommends a ratio of blood to anticoagulant volume of 9:1. However, investigators have published reports which suggest that a lower ratio may be acceptable. Unfortunately the recommendations of these reports are inconsistent. AIM: The aim of this study was to determine the impact of tube fill volume on INR values both in healthy subjects and patients receiving oral anticoagulation therapy. METHODS: INR values were obtained by processing coagulation specimens containing different volumes of whole blood. The study group included 30 patients taking oral anticoagulation therapy and 15 healthy volunteers. Respectively 2.5ml, 3 ml, 3.5 ml, 4 ml and 4.5 ml of whole blood was drawn into tubes containing a fixed volume of 3.2% (0.109M) sodium citrate. RESULTS: The INR values increased as total tube fill volumes decreased for both groups but this finding did not reach statistical significance in either group for the tube fill volumes studied. CONCLUSION: For blood specimens collected in 3.2% citrate anticoagulant, a total tube fill volume of greater than 56% yielded reliable INR results.en_US
dc.description.urihttp://www.smltsa.org.zaen_US
dc.identifier.citationPotgieter, JJC, Pool, R, Prinsloo, A, Botha, EMD & Olorunju, S 2010, 'The impact of collection tube fill volume on international normalized ratio', Medical Technology SA, vol. 24, no. 1, pp. 11-16.en_US
dc.identifier.issn1011-5528
dc.identifier.urihttp://hdl.handle.net/2263/17224
dc.language.isoenen_US
dc.publisherSociety of Medical Laboratory Technologists of South Africaen_US
dc.rightsSociety of Medical Laboratory Technologists of South Africaen_US
dc.subjectTube fill volumeen_US
dc.subjectInternational normalized ratio (INR)en_US
dc.subject.lcshBlood -- Collection and preservation -- Researchen
dc.titleThe impact of collection tube fill volume on international normalized ratioen_US
dc.typeArticleen_US

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