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Rapid detection of equine piroplasms using multiplex PCR and first genetic characterization of Theileria haneyi in Egypt

dc.contributor.authorElsawy, Bassma S.M.
dc.contributor.authorNassar, Ahmed M.
dc.contributor.authorAlzan, Heba F.
dc.contributor.authorBhoora, Raksha Vasantrai
dc.contributor.authorOzubek, Sezayi
dc.contributor.authorMahmoud, Mona S.
dc.contributor.authorKandil, Omnia M.
dc.contributor.authorMahdy, Olfat A.
dc.date.accessioned2022-02-21T13:02:12Z
dc.date.available2022-02-21T13:02:12Z
dc.date.issued2021-10-31
dc.description.abstractEquine Piroplasmosis (EP) is an infectious disease caused by the hemoprotozoan parasites Theileria equi, Babesia caballi, and the recently identified species T. haneyi. Hereby, we used a multiplex PCR (mPCR) targeting the 18S rRNA gene of T. equi and B. caballi for the simultaneous detection of EP in Egyptian equids and examined the presence of T. haneyi infections in Egypt. Blood samples from 155 equids (79 horses and 76 donkeys) collected from different governorates of Egypt were examined by mPCR and PCR targeting T. hayeni. The mPCR method revealed a prevalence of T. equi of 20.3% in horses and of 13.1% in donkeys and a prevalence of B. caballi of 1.2% in horses. B. caballi was not detected in donkeys in the current study. The mPCR method also detected coinfections with both species (2.5% and 1.3% in horses and donkeys, respectively). Additionally, we report the presence of T. haneyi in Egypt for the first time in 53.1% of the horse and 38.1% of the donkey tested samples. Coinfection with T. haneyi and T. equi was found in 13.5% of the samples, while infection with the three EP species was found in 1.9% of the samples.en_ZA
dc.description.departmentVeterinary Tropical Diseasesen_ZA
dc.description.librarianpm2022en_ZA
dc.description.sponsorshipB.S.M.E, National research center, (NRC)en_ZA
dc.description.urihttp://www.mdpi.com/journal/pathogensen_ZA
dc.identifier.citationElsawy, B.S.M.; Nassar, A.M.; Alzan, H.F.; Bhoora, R.V.; Ozubek, S.; Mahmoud, M.S.; Kandil, O.M.; Mahdy, O.A. Rapid Detection of Equine Piroplasms Using Multiplex PCR and First Genetic Characterization of Theileria haneyi in Egypt. Pathogens 2021, 10, 1414. https://doi.org/10.3390/pathogens10111414.en_ZA
dc.identifier.issn2076-0817 (online)
dc.identifier.other10.3390/ pathogens10111414
dc.identifier.urihttp://hdl.handle.net/2263/84086
dc.language.isoenen_ZA
dc.publisherMDPIen_ZA
dc.rights© 2021 by the authors. Licensee: MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).en_ZA
dc.subjectEquidsen_ZA
dc.subjectBabesia caballien_ZA
dc.subjectTheileria equien_ZA
dc.subjectTheileria haneyien_ZA
dc.subject18S rRNA gene and sequencingen_ZA
dc.subjectMultiplex PCR (mPCR)en_ZA
dc.subjectPolymerase chain reaction (PCR)en_ZA
dc.subjectConventional PCR (cPCR)en_ZA
dc.titleRapid detection of equine piroplasms using multiplex PCR and first genetic characterization of Theileria haneyi in Egypten_ZA
dc.typeArticleen_ZA

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