Antioxidant supplementation mitigates DNA damage in boar (Sus scrofa domesticus) spermatozoa induced by tropical summer

dc.contributor.authorPeña, Santiago T. Jr.
dc.contributor.authorGummow, Bruce
dc.contributor.authorParker, Anthony J.
dc.contributor.authorParis, Damien B.B.P.
dc.date.accessioned2019-09-18T07:39:17Z
dc.date.available2019-09-18T07:39:17Z
dc.date.issued2019-04-30
dc.description.abstractHeat stress-induced sperm DNA damage has recently been demonstrated in boars during tropical summer; which could negatively impact early embryo survival and litter size in sows. Given the boar’s inefficient capacity to sweat, non-pendulous scrotum and low antioxidant activity in seminal plasma, elevated endogenous levels of antioxidants are needed to combat reactive oxygen species induced during periods of heat stress. This should prevent the build-up of pathological levels of DNA damage in boar spermatozoa. Our aim was to investigate whether a combined antioxidant supplement could mitigate sperm DNA damage in boars exposed to tropical summer conditions. Terminal deoxynucleotidyl transferase dUTP nick end labelling and flow cytometry of 20,000 spermatozoa/boar/treatment revealed that boar diets supplemented with 100 g/day custom-mixed antioxidant during peak wet summer effectively reduced sperm DNA damage by as much as 55% after 42 and 84 days treatment respectively (16.1 ± 4.9 peak wet control vs. 9.9 ± 4.5 42 day vs. 7.2 ± 1.6% 84 day treatments; P 0.05). Supplementation did not improve sperm concentration beyond control levels for either season (P > 0.05); nor alter total motility, progressive motility or several other motion parameters measured by computer assisted sperm analysis of 20 x 106 sperm/mL at 38˚C (P > 0.05). Antioxidant supplementation during tropical summer appears to mitigate the negative impact of heat stress on DNA integrity but not concentration nor motility of boar spermatozoa; which may provide one solution to the problem of summer infertility in the pig.en_ZA
dc.description.departmentProduction Animal Studiesen_ZA
dc.description.librarianam2019en_ZA
dc.description.sponsorshipA JCU Development Grant to DP, and College of Public Health, Medical and Veterinary Sciences PhD Research Funds to SP. SP was also supported by the Australia Awards Scholarship.en_ZA
dc.description.urihttps://journals.plos.org/plosoneen_ZA
dc.identifier.citationPeña ST, Jr., Gummow B, Parker AJ, Paris DBBP (2019) Antioxidant supplementation mitigates DNA damage in boar (Sus scrofa domesticus) spermatozoa induced by tropical summer. PLoS ONE 14(4): e0216143. https://DOI.org/10.1371/journal.pone.0216143.en_ZA
dc.identifier.issn1932-6203 (online)
dc.identifier.other10.1371/journal.pone.0216143
dc.identifier.urihttp://hdl.handle.net/2263/71393
dc.language.isoenen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.rights© 2019 The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License.en_ZA
dc.subjectDNA damageen_ZA
dc.subjectTropical summeren_ZA
dc.subjectHeat stressen_ZA
dc.subjectBoar (Sus scrofa)en_ZA
dc.subjectOxidative stressen_ZA
dc.subjectAmbient temperatureen_ZA
dc.subjectDietary seleniumen_ZA
dc.subjectSemen qualityen_ZA
dc.subjectVitamin Een_ZA
dc.subjectReproductive performanceen_ZA
dc.subjectMale infertilityen_ZA
dc.subjectSpermen_ZA
dc.subjectIn vivoen_ZA
dc.subjectFolic aciden_ZA
dc.titleAntioxidant supplementation mitigates DNA damage in boar (Sus scrofa domesticus) spermatozoa induced by tropical summeren_ZA
dc.typeArticleen_ZA

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