Profiling anticancer and antioxidant activities of phenolic compounds present in black walnuts (Juglans nigra) using a high-throughput screening approach

dc.contributor.authorHo, Khanh-Van
dc.contributor.authorRoy, Anuradha
dc.contributor.authorFoote, Sarah
dc.contributor.authorVo, Phuc H.
dc.contributor.authorLall, Namrita
dc.contributor.authorLin, Chung-Ho
dc.date.accessioned2020-12-23T07:57:19Z
dc.date.available2020-12-23T07:57:19Z
dc.date.issued2020-10
dc.descriptionSupplementary Materials: Figure S1: Data distribution of controls (Trolox, DL-sulforaphane, tert- butylhydroquinone) in total antioxidant capacity and antioxidant response element (ARE) activation assays. Figure S2: Data distribution of controls (Trolox, DL-sulforaphane) in cytotoxicity assays.en_ZA
dc.description.abstractOur recent studies have demonstrated multiple health-promoting benefits from black walnut kernels. These biological functions of black walnuts are likely associated with their bioactive constituents. Characterization of phenolic compounds found in black walnut could point out underexplored bioactive activities of black walnut extracts and promote the development of novel applications of black walnut and its by-products. In the present study, we assessed bioactivity profiles of phenolic compounds identified in the kernels of black walnuts using a high-throughput screening (HTS) approach. Black walnut phenolic compounds were evaluated in terms of their total antioxidant capacity, antioxidant response element (ARE) induction, and anticancer activities. The anticancer activities were identified by evaluating the effects of the phenolic compounds on the growth of the tumorigenic alveolar epithelial cells (A549) and non-tumorigenic lung fibroblast cells (MRC-5). Out of 16 phenolic compounds tested, several compounds (penta-O-galloyl-β-d-glucose, epicatechin gallate, quercetin, (–)-epicatechin, rutin, quercetin 3-β-d-glucoside, gallic acid, (+)-catechin, ferulic acid, syringic acid) exerted antioxidant activities that were significantly higher compared to Trolox, which was used as a control. Two phenolic compounds, penta-O-galloyl-β-d-glucose and quercetin 3-β-d-glucoside, exhibited antiproliferative activities against both the tumorigenic alveolar epithelial cells (A549) and non-tumorigenic lung fibroblast cells (MRC-5). The antioxidant activity of black walnut is likely driven not only by penta-O-galloyl-β-d-glucose but also by a combination of multiple phenolic compounds. Our findings suggested that black walnut extracts possibly possess anticancer activities and supported that penta-O-galloyl-β-d-glucose could be a potential bioactive agent for the cosmetic and pharmaceutical industries.en_ZA
dc.description.departmentPlant Production and Soil Scienceen_ZA
dc.description.librarianpm2020en_ZA
dc.description.sponsorshipUSDA/ARS Dale Bumpers Small Farm Research Center, Center for Agroforestry at University of Missouri and Missouri Department of Agriculture Specialty Crop Block Grant Program (SCBGP).en_ZA
dc.description.urihttp://www.mdpi.com/journal/moleculesen_ZA
dc.identifier.citationHo, K-V., Roy, A., Foote, S. et al. 2020, 'Profiling anticancer and antioxidant activities of phenolic compounds present in black walnuts (Juglans nigra) using a high-throughput screening approach', Molecules, vol. 25, no. 19, art. 4516, pp. 1-13.en_ZA
dc.identifier.issn1420-3049 (online)
dc.identifier.other10.3390/molecules25194516
dc.identifier.urihttp://hdl.handle.net/2263/77491
dc.language.isoenen_ZA
dc.publisherMDPIen_ZA
dc.rights© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en_ZA
dc.subjectPenta-O-galloyl-β-d-glucoseen_ZA
dc.subjectPolyphenolen_ZA
dc.subjectAntioxidant response elementen_ZA
dc.subjectAnticanceren_ZA
dc.subjectBlack walnuts (Juglans nigra)en_ZA
dc.subjectAntioxidant activityen_ZA
dc.subjectHigh-throughput screening (HTS)en_ZA
dc.titleProfiling anticancer and antioxidant activities of phenolic compounds present in black walnuts (Juglans nigra) using a high-throughput screening approachen_ZA
dc.typeArticleen_ZA

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