Demonstration of lumpy skin disease virus infection in Amblyomma hebraeum and Rhipicephalus appendiculatus ticks using immunohistochemistry

dc.contributor.authorLubinga, Jimmy Clement
dc.contributor.authorClift, Sarah Jane
dc.contributor.authorTuppurainen, Eeva S.M.
dc.contributor.authorStoltsz, Wilhelm Heinrich
dc.contributor.authorBabiuk, Shawn
dc.contributor.authorCoetzer, Jacobus A.W.
dc.contributor.authorVenter, Estelle Hildegard
dc.date.accessioned2014-05-21T10:02:04Z
dc.date.available2014-05-21T10:02:04Z
dc.date.issued2014-03
dc.description.abstractLumpy skin disease (LSD) is caused by lumpy skin disease virus (LSDV), a member of the genus Capripoxvirus. Transmission of the virus has been associated with haematophagous insects such as Stomoxys calcitrans as well as Aedes and Culex species of mosquitoes. Recent studies have reported the transmission of the virus by Amblyomma hebraeum, Rhipicephalus appendiculatus, and Rhipicephalus decoloratus ticks and the presence of LSDV in saliva of A. hebraeum and R. appendiculatus ticks. The aim of this study was to determine which tick organs become infected by LSDV following intrastadial infection and transstadial persistence of the virus in A. hebraeum and R. appendiculatus ticks. Nymphal and adult ticks were orally infected by feeding them on LSDV-infected cattle. Partially fed adult ticks were processed for testing while nymphs were fed to repletion and allowed to moult to adults before being processed for testing. The infection in tick organs was determined by testing for the presence of the viral antigen using monoclonal antibodies with immunohistochemical staining. The viral antigen was detected in salivary glands, haemocytes, synganglia, ovaries, testes, fat bodies, and midgut. Since the virus was shown to be able to cross the midgut wall and infect various tick organs, this may indicate potential for biological development and transmission of LSDV in ticks. This study strengthens the previously reported evidence of the occurrence of LSDV in tick saliva.en
dc.description.librarianhb2014en
dc.description.librarianab2014
dc.description.urihttp://www.elsevier.com/locate/ttbdisen
dc.identifier.citationLubinga, JC, Clift, SJ,Tuppurainen, ESM, Stoltsz, WH, Babiuk, S, Coetzer, JAW & Venter, EH 2014, 'Demonstration of lumpy skin disease virus infection in Amblyomma hebraeum and Rhipicephalus appendiculatus ticks using immunohistochemistry', Ticks and Tick-borne Diseases, vol. 5, no. 2, pp. 113-120.en
dc.identifier.issn1877-959X (print)
dc.identifier.issn1877-9603 (online)
dc.identifier.other10.1016/j.ttbdis.2013.09.010
dc.identifier.other7003904650
dc.identifier.otherO-6953-2014
dc.identifier.urihttp://hdl.handle.net/2263/39835
dc.language.isoenen
dc.publisherElsevieren
dc.relation.requiresAdobe Acrobat Readeren
dc.rights© 2013 Elsevier GmbH. All rights reserved.Notice : this is the author’s version of a work that was accepted for publication in Ticks and Tick-borne Diseases. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Ticks and Tick-borne Diseases, vol. 5, no. 2, pp. 113-120, 2014. doi : 10.1016/j.ttbdis.2013.09.010en
dc.subjectTransstadial passageen
dc.subjectAmblyomma hebraeumen
dc.subjectIntrastadial infectionen
dc.subjectLSDVen
dc.subject.lcshLumpy skin disease virusen
dc.subject.lcshAmblyommaen
dc.subject.lcshRhipicephalus appendiculatusen
dc.subject.lcshTicksen
dc.subject.lcshImmunohistochemistryen
dc.titleDemonstration of lumpy skin disease virus infection in Amblyomma hebraeum and Rhipicephalus appendiculatus ticks using immunohistochemistryen
dc.typePostprint Articleen

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