Optimisation of histocompatibility testing in living-donor renal transplantation

dc.contributor.advisorMeyer, Pieter
dc.contributor.coadvisorKarusseit, V.O.L.
dc.contributor.emailluyanda.kwofie@nhls.ac.zaen_ZA
dc.contributor.postgraduateKwofie, Luyanda Laura Illicia
dc.date.accessioned2019-02-14T12:51:10Z
dc.date.available2019-02-14T12:51:10Z
dc.date.created2019-04
dc.date.issued2018
dc.descriptionThesis (PhD)--University of Pretoria, 2018.en_ZA
dc.description.abstractThe study addressed the development and evaluation of novel procedures for the rapid, reproducible and efficient measurement of T cell alloreactivity both pre- and post-transplantation, with the aim of optimizing donor/ recipient compatibility in renal transplantation. Furthermore, a comparison of the three currently available procedures for the detection of cytotoxic antibodies to determine which of these is best suited to optimizing pre-transplantation donor-recipient matching, as well as post-transplantation detection of sensitization to donor alloantigens. She established that the standard CDC crossmatching (CDCXM) had a high false-positive rate and that the Donor specific antigen crossmatch (DSAXM) method delivered the most accurate results for identifying anti-HLA antibodies in prospective, pre-sensitised kidney transplant patients. Cytokine profile analysis identified the involvement of eosinophils and a marked increase of anti-inflammatory cytokines that may possibly contribute to the initiation of kidney transplant rejection. Finally, she has shown that, the use of novel, CFSE-based two-way mixed lymphocyte cultures (MLC) adapted for flow cytometry may be able to identify patients that are at risk of transplant rejection. Furthermore, this novel method may possibly be the test of choice to indicate probable graft-versus-host-disease (GVHD) incidences. The overall findings of the study may contribute to optimising histocompatibility testing in renal transplantation by improving HLA matching criteria as well as identifying potential strategies to monitor and prevent GVHD.en_ZA
dc.description.availabilityUnrestricteden_ZA
dc.description.degreePhDen_ZA
dc.description.departmentImmunologyen_ZA
dc.description.sponsorshipNational Health Laboratory Services Research Trusten_ZA
dc.identifier.citationKwofie, LLI 2018, Optimisation of histocompatibility testing in living-donor renal transplantation, PhD Thesis, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/68460>en_ZA
dc.identifier.otherA2019en_ZA
dc.identifier.urihttp://hdl.handle.net/2263/68460
dc.language.isoenen_ZA
dc.publisherUniversity of Pretoria
dc.rights© 2018 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.
dc.subjectHLA cross-matchen_ZA
dc.subjectHistocompatibilityen_ZA
dc.subjectEnd-stage kidney dieaseen_ZA
dc.subjectAllotransplantationen_ZA
dc.subjectAlloantigenen_ZA
dc.subjectT cell alloreactivity
dc.subjectRenal transplantation
dc.subjectDonor-recipient compatibility
dc.subjectKidney transplant
dc.subjectCytotoxic antibodies
dc.subjectCDC crossmatching
dc.subjectDonor-specific antigen crossmatch
dc.subject.otherHealth sciences theses SDG-03
dc.subject.otherSDG-03: Good health and well-being
dc.subject.otherHealth sciences theses SDG-10
dc.subject.otherSDG-10: Reduced inequalities
dc.subject.otherHealth sciences theses SDG-17
dc.subject.otherSDG-17: Partnerships for the goals
dc.titleOptimisation of histocompatibility testing in living-donor renal transplantationen_ZA
dc.typeThesisen_ZA

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Kwofie_Optimisation_2018.pdf
Size:
4.2 MB
Format:
Adobe Portable Document Format
Description:
Dissertation

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description: