89Zr-Leukocyte labelling for cell trafficking : in vitro and preclinical investigations
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Date
Authors
Kahts, Maryke
Guo, Hua
Kommidi, Harikrishna
Yang, Yanping
Sayman, Haluk Burcak
Summers, Beverley
Ting, Richard
Zeevaart, Jan Rijn
Sathekge, Mike Machaba
Aras, Omer
Journal Title
Journal ISSN
Volume Title
Publisher
SpringerOpen
Abstract
BACKGROUND : The non-invasive imaging of leukocyte trafficking to assess inflammatory
areas and monitor immunotherapy is currently generating great interest. There
is a need to develop more robust cell labelling and imaging approaches to track
living cells. Positron emission tomography (PET), a highly sensitive molecular imaging
technique, allows precise signals to be produced from radiolabelled moieties. Here,
we developed a novel leukocyte labelling approach with the PET radioisotope zirconium-
89 (89Zr, half-life of 78.4 h). Experiments were carried out using human leukocytes,
freshly isolated from whole human blood.
RESULTS : The 89Zr-leukocyte labelling efficiency ranged from 46 to 87%
after 30–60 min. Radioactivity concentrations of labelled cells were up to 0.28 MBq/1
million cells. Systemically administered 89Zr-labelled leukocytes produced highcontrast
murine PET images at 1 h–5 days post injection. Murine biodistribution data
showed that cells primarily distributed to the lung, liver, and spleen at 1 h post injection,
and are then gradually trafficked to liver and spleen over 5 days. Histological
analysis demonstrated that exogenously 89Zr-labelled human leukocytes were present
in the lung, liver, and spleen at 1 h post injection. However, intravenously injected
free [
89Zr]Zr4+ ion showed retention only in the bone with no radioactivity in the lung
at 5 days post injection, which implied good stability of radiolabelled leukocytes
in vivo.
CONCLUSIONS : Our study presents a stable and generic radiolabelling technique
to track leukocytes with PET imaging and shows great potential for further applications
in inflammatory cell and other types of cell trafficking studies.
Description
ADDITIONAL FILE 1 : PET/CT images showing that intravenously introduced 89Zr-labelled leukocytes accumulated in
the lung at 1 day and 5 days post injection, with slight migration to the liver over time. Free zirconium-89 accumulated
in bone with no lung uptake. 5-week-old female Balb/c mice were intravenously injected with 0.22 MBq
89Zr-labelled leukocytes or free [
89Zr]Zr4+ ion (control) and 30 min PET scans were performed at 1 day and 5 days
post injection.
DATA AVAILABILITY STATEMENT : The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.
DATA AVAILABILITY STATEMENT : The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.
Keywords
Cell trafficking, Infection imaging, Inflammation, Zirconium-89, Positron emission tomography (PET), SDG-03: Good health and well-being
Sustainable Development Goals
SDG-03:Good heatlh and well-being
Citation
Kahts, M., Guo, H., Kommidi, H. et al. 2023, '89Zr-Leukocyte labelling for cell trafficking : in vitro and preclinical investigations', EJNMMI Radiopharmacy and Chemistry, vol. 8, no. 36, pp. 1-16. https://DOI.org/10.1186/s41181-023-00223-1.