Transcriptome analysis of the inflammatory responses of bovine mammary epithelial cells : exploring immunomodulatory target genes for bovine mastitis

dc.contributor.authorIslam, Md.Aminul
dc.contributor.authorTakagi, Michihiro
dc.contributor.authorFukuyama, Kohtaro
dc.contributor.authorKomatsu, Ryoya
dc.contributor.authorAlbarracin, Leonardo
dc.contributor.authorNochi, Tomonori
dc.contributor.authorSuda, Yoshihito
dc.contributor.authorIkeda-Ohtsubo, Wakako
dc.contributor.authorRutten, Victor P.M.G.
dc.contributor.authorVan Eden, Willem
dc.contributor.authorVillena, Julio
dc.contributor.authorAso, Hisashi
dc.contributor.authorKitazawa, Haruki
dc.date.accessioned2021-10-26T08:39:29Z
dc.date.available2021-10-26T08:39:29Z
dc.date.issued2020-03-09
dc.description.abstractBovine mastitis is the inflammatory reaction of the mammary gland and is commonly caused by bacterial infections in high-yielding dairy cows. The detailed investigation of the immunotranscriptomic response of bovine mammary epithelial (BME) cells to pattern recognition receptors (PRRs) activation by microbial-associated molecular patterns (MAMPs) can be of great importance for understanding the innate immune defense mechanisms, and for exploring the immunomodulatory candidate genes. In this work, we investigated the transcriptome modifications of BME cells after the in vitro stimulation with Escherichia coli derived lipopolysaccharide (LPS) and heat-killed Staphylococcus aureus JE2 and S. aureus SA003. In addition, the effect of Pam3CSK4 (a synthetic triacylated lipopeptide that activates Toll-like receptor 2 (TLR2)), and the intracellular chemotactic protein cyclophilin A (CyPA), which is secreted by BME cells during mastitis, in the expression changes of selected cytokines and chemokines were evaluated by qPCR. Microarray analysis identified 447, 465 and 520 differentially expressed genes (DEGs) in the BME cells after LPS, S. aureus JE2 and S. aureus SA003 stimulation, respectively. A major differential response in the inflammatory gene expression was noticed between the stimulation of LPS and S. aureus strains. Unlike the S. aureus strains, LPS stimulation resulted in significant upregulation of CCL2, CXCL2, CXCL3, CXCL8, IL1 and IL1 , which were confirmed by qPCR analysis. Pam3CSK4 was not able to induce significant changes in the expression of cytokines and chemokines in challenged BME cells. The exogenous CyPA administration was able to upregulate CXCL2, CXCL3, CXCL8, IL1 and IL1 expression in BME cells indicating its ability to promote inflammation. The identification of transcriptional markers of mastitis specific for individual inflammatory factors such as LPS, Pam3CSK4 or CyPA, which can be evaluated in vitro in BME cells, may enable the development of novel diagnostics and/or immunomodulatory treatments, providing new tools for the effective management of mastitis in dairy cows. The results of this work are an advance in this regard.en_ZA
dc.description.departmentVeterinary Tropical Diseasesen_ZA
dc.description.librarianam2021en_ZA
dc.description.sponsorshipThe project of NARO Bio-oriented Technology Research Advancement Institution (Research Program on development of innovation technology, No. 29006A), a Grant-in-Aid for Scientific Research (A) (19H00965), (B) (16H05019), Challenging Exploratory Research (26660216, 16K15028), Open Partnership Joint Projects of JSPS Bilateral Joint Research Projects from the Japan Society for the Promotion of Science (JSPS), JSPS (Postdoctoral Fellowship for Foreign Researchers, Program No. 18F18081).en_ZA
dc.description.urihttp://www.mdpi.com/journal/pathogensen_ZA
dc.identifier.citationIslam, M.A; Takagi, M; Fukuyama, K. et al. 2020, 'Transcriptome analysis of the inflammatory responses of bovine mammary epithelial cells : exploring immunomodulatory target genes for bovine mastitis', Pathogens, vol. 9, no. 200, pp. 1-18.en_ZA
dc.identifier.issn2076-0817 (online)
dc.identifier.other10.3390/pathogens9030200
dc.identifier.urihttp://hdl.handle.net/2263/82249
dc.language.isoenen_ZA
dc.publisherMDPIen_ZA
dc.rights© 2020 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en_ZA
dc.subjectLPSen_ZA
dc.subjectTLR2en_ZA
dc.subjectTLR4en_ZA
dc.subjectTranscriptomeen_ZA
dc.subjectInflammationen_ZA
dc.subjectCyclophilin Aen_ZA
dc.subjectBovine mammary epithelial (BME)en_ZA
dc.subjectBovine mastitisen_ZA
dc.subjectDairy cowsen_ZA
dc.subjectPattern recognition receptors (PRRs)en_ZA
dc.subjectMicrobial-associated molecular pattern (MAMP)en_ZA
dc.titleTranscriptome analysis of the inflammatory responses of bovine mammary epithelial cells : exploring immunomodulatory target genes for bovine mastitisen_ZA
dc.typeArticleen_ZA

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