Exploring early steps in biofilm formation : set-up of an experimental system for molecular studies

dc.contributor.authorCrouzet, Marc
dc.contributor.authorLe Senechal, Caroline
dc.contributor.authorBrözel, Volker Siegfried
dc.contributor.authorCostaglioli, Patricia
dc.contributor.authorBarthe, Christophe
dc.contributor.authorBonneu, Marc
dc.contributor.authorGarbay, Bertrand
dc.contributor.authorVilain, Sebastien
dc.date.accessioned2014-12-01T07:52:04Z
dc.date.available2014-12-01T07:52:04Z
dc.date.issued2014-09-30
dc.description.abstractBACKGROUND: Bacterial biofilms are predominant in natural ecosystems and constitute a public health threat because of their outstanding resistance to antibacterial treatments and especially to antibiotics. To date, several systems have been developed to grow bacterial biofilms in order to study their phenotypes and the physiology of sessile cells. Although relevant, such systems permit analysis of various aspects of the biofilm state but often after several hours of bacterial growth. RESULTS: Here we describe a simple and easy-to-use system for growing P. aeruginosa biofilm based on the medium adsorption onto glass wool fibers. This approach which promotes bacterial contact onto the support, makes it possible to obtain in a few minutes a large population of sessile bacteria. Using this growth system, we demonstrated the feasibility of exploring the early stages of biofilm formation by separating by electrophoresis proteins extracted directly from immobilized cells. Moreover, the involvement of protein synthesis in P. aeruginosa attachment is demonstrated. CONCLUSIONS: Our system provides sufficient sessile biomass to perform biochemical and proteomic analyses from the early incubation period, thus paving the way for the molecular analysis of the early stages of colonization that were inaccessible to date.en_US
dc.description.librarianam2014en_US
dc.description.sponsorshipThe Institut Polytechnique de Bordeaux and the University Bordeaux Segalen.en_US
dc.description.urihttp://www.biomedcentral.com/bmcmicrobiol/en_US
dc.identifier.citationCrouzet, M, Le Senechal, C, Brözel, VS, Costaglioli, P, Barthe, C, Bonneu, M, Garbay, B & Vilain, S 2014, 'Exploring early steps in biofilm formation : set-up of an experimental system for molecular studies', BMC Microbiology, vol. 14, art. 253, pp. 1-12.en_US
dc.identifier.issn1471-2180
dc.identifier.other10.1186/s12866-014-0253-z
dc.identifier.urihttp://hdl.handle.net/2263/42721
dc.language.isoenen_US
dc.publisherBioMed Centralen_US
dc.rights© 2014 Crouzet et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.en_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjectGlass woolen_US
dc.subjectAdsorption modeen_US
dc.subjectAdhesionen_US
dc.subjectBiofilmen_US
dc.titleExploring early steps in biofilm formation : set-up of an experimental system for molecular studiesen_US
dc.typeArticleen_US

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