Diversity, phylogeny and pathogenicity of Botryosphaeriaceae on nonnative Eucalyptus grown in an urban environment : a case study

dc.contributor.authorPavlic-Zupanc, Draginja
dc.contributor.authorMaleme, Happy-girl Mamodise
dc.contributor.authorWingfield, Brenda D.
dc.contributor.authorWingfield, Michael J.
dc.contributor.authorSlippers, Bernard
dc.date.accessioned2017-10-25T11:04:58Z
dc.date.issued2017-08
dc.description.abstractThe Botryosphaeriaceae are opportunistic pathogens mostly of woody plants, including Eucalyptus. These fungi can cause cankers and die-back diseases on non-native Eucalyptus trees in South African plantations. Botryosphaeriaceae were isolated from diseased and asymptomatic twigs and leaves from 20 Eucalyptus spp. grown in a Pretoria, South Africa arboretum and its surroundings. The isolates were initially grouped based on conidial morphology and Internal Transcribed Spacer (ITS) rDNA PCR-RFLP profiles. They were further identified using DNA sequence data for the ITS rDNA and translation elongation factor 1-α (TEF-1α) gene regions and tested for pathogenicity. Five species were identified including Botryosphaeria dothidea and four Neofusicoccum species namely Neofusicoccum parvum; N. cryptoaustrale and N. ursorum that were recently described from plant tissues collected as a part of the current study; and Neofusicoccum eucalypti (Winter) Maleme, Pavlic & Slippers comb. nov. The latter species is recorded for the first time on Eucalyptus in South Africa. Most of the identified species were collected from the leaves of 17 different Eucalyptus spp. Neofusicoccum parvum was most commonly isolated (72% of all isolates) followed by B. dothidea species complex (17%). With exception of N. parvum which was isolated from majority of Eucalyptus spp. the other species were isolated from limited number of Eucalyptus species indicating host-preferences. All the isolated Botryosphaeriaceae species produced lesions on inoculated Eucalyptus grandis plants that were significantly larger than those associated with the controls.en_ZA
dc.description.departmentForestry and Agricultural Biotechnology Institute (FABI)en_ZA
dc.description.departmentGeneticsen_ZA
dc.description.departmentMicrobiology and Plant Pathologyen_ZA
dc.description.embargo2018-08-30
dc.description.librarianhj2017en_ZA
dc.description.sponsorshipThe National Research Foundation (NRF), members of the Tree Protection Co-operative Programme (TPCP), Department of Science and technology (DST/NRF Centre of Excellence in Tree Health Biotechnology (CTHB), South Africa, Department of Water affairs and Forestry, South Africa.en_ZA
dc.description.urihttp://www.elsevier.com/locate/ufugen_ZA
dc.identifier.citationPavlic-Zupanc, D., Maleme, H.M., Piskur, B., Wingfield, B.D., Wingfield, M.J. & Slippers, B. 2017, 'Diversity, phylogeny and pathogenicity of Botryosphaeriaceae on nonnative Eucalyptus grown in an urban environment : a case study', Urban Forestry and Urban Greening, vol. 26, pp. 139-148.en_ZA
dc.identifier.issn1618-8667 (print)
dc.identifier.issn1610-8167 (online)
dc.identifier.other10.1016/j.ufug.2017.04.009
dc.identifier.urihttp://hdl.handle.net/2263/62933
dc.language.isoenen_ZA
dc.publisherElsevieren_ZA
dc.rights© 2017 Elsevier GmbH. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Urban Forestry and Urban Greening. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Urban Forestry and Urban Greening, vol. 26, pp. 139-148, 2017. doi : 10.1016/j.ufug.2017.04.009.en_ZA
dc.subjectDie-backen_ZA
dc.subjectEndophytesen_ZA
dc.subjectLatent pathogenen_ZA
dc.subjectPathogenicityen_ZA
dc.subjectUrban habitaten_ZA
dc.subjectComplexen_ZA
dc.subjectDiseaseen_ZA
dc.subjectTreesen_ZA
dc.subjectCankeren_ZA
dc.subjectWestern Australiaen_ZA
dc.subjectEucalyptus plantationsen_ZA
dc.subjectSouth Africa (SA)en_ZA
dc.subjectNative Syzygium cordatumen_ZA
dc.titleDiversity, phylogeny and pathogenicity of Botryosphaeriaceae on nonnative Eucalyptus grown in an urban environment : a case studyen_ZA
dc.typePostprint Articleen_ZA

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