Toxorhynchites-fluorescent antibody system for the detection of bluetongue virus from Culicoides midges (Diptera: Ceratopogonidae)

dc.contributor.authorHabibur Rahman, A.
dc.contributor.authorManickam, R.
dc.contributor.editorVerwoerd, Daniel Wynand
dc.date.accessioned2012-12-12T08:50:10Z
dc.date.available2012-12-12T08:50:10Z
dc.date.created2012
dc.date.issued1997
dc.descriptionThe articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.en_US
dc.description.abstractA new system, the Toxorhynchites-fluorescent antibody (TFA) test in which the larvae of Toxorhynchites splendens mosquitoes were used for the detection of bluetongue virus (BTV) from Culicoides midges, was developed. Twenty-seven pools of Culicoides midges were collected from bluetongue-prone areas of Tamil Nadu by use of the light-trap and suction-trap methods. A suspension of each pool was injected intrathoracically into T. splendens IV instar larvae and inoculated onto Vero cell monolayers. An indirect fluorescent antibody technique and an immunoperoxidase test were used to detect BTV antigen in smears of crushed midges, crushed larval head smears after incubation for 7 d at 28° and cell monolayers showing cytopathic effects 48 h post inoculation. The suspensions were also injected intravenously into embryonated chicken eggs, and the characteristic BTV-induced lesion(s), viz. cherry-red appearance of embryos, were observed after 48 h. Virus was confirmed by a qualitative neutralization test conducted simultaneously in embryonated chicken eggs. A total of seven out of 27 samples (26%) were positive for the presence of BTV antigen in all the diagnostic systems used. Since BTV propagates readily in experimentally infected T. splendens larvae and the BTV antigen can be detected by the fluorescent antibody technique with a sensitivity comparable to that for virus propagated in tissue culture and embryonated eggs, the TFA system can be adopted as a new method for the isolation of BTV from vectors. The advantages of the TFA system are discussed.en_US
dc.identifier.citationHabibur Rahman, A & Manickam, R 1997, 'Toxorhynchites-fluorescent antibody system for the detection of bluetongue virus from Culicoides midges (Diptera: Ceratopogonidae)’. Onderstepoort Journal of Veterinary Research, vol. 64, no. 4, pp. 301-307.en_US
dc.identifier.issn0330-2465
dc.identifier.urihttp://hdl.handle.net/2263/20745
dc.language.isoenen_US
dc.publisherPublished by the Agricultural Research Council, Onderstepoort Veterinary Instituteen_US
dc.rights© ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital).en_US
dc.subjectVeterinary medicineen_US
dc.subjectBluetongue virusen_US
dc.subjectCulicoidesen_US
dc.subjectImmunoperoxidase testen_US
dc.subjectIndirect fluorescent antibody techniqueen_US
dc.subjectLarvaeen_US
dc.subjectMosquitoen_US
dc.subjectToxorhynchites splendensen_US
dc.subject.lcshVeterinary medicine -- South Africa
dc.titleToxorhynchites-fluorescent antibody system for the detection of bluetongue virus from Culicoides midges (Diptera: Ceratopogonidae)en_US
dc.typeArticleen_US

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