Loop-mediated isothermal amplification (LAMP) detection of Babesia orientalis in water buffalo (Bubalus babalis, Linnaeus, 1758) in China

dc.contributor.authorHe, Lan
dc.contributor.authorZhou, Yan-Qin
dc.contributor.authorOosthuizen, Marinda C.
dc.contributor.authorZhao, Jun-Long
dc.contributor.emailmarinda.Oosthuizen@up.ac.zaen
dc.date.accessioned2009-10-28T10:57:31Z
dc.date.available2009-10-28T10:57:31Z
dc.date.issued2009
dc.description.abstractLoop-mediated isothermal amplification (LAMP) is a rapid method with high specificity and efficiency under isothermal condition using a set of four specifically designed primers that recognize six distinct sequences on the target gene. In this study, a LAMPmethod was developed for specific detection of Babesia orientalis in water buffalo (Bubalus babalis, Linnaeus, 1758). Four primers were designed from the V4 hypervariable region of the 18S rRNA gene of B. orientalis. Blood samples were collected from B. orientalis experimentally infected water buffalo as well as from 165 water buffalo from eight different regions of the Hubei province, south China. Genomic DNA was extracted, subjected to the LAMP assay and compared with results obtained using a previously described semi-nested PCR. The LAMP assay proofed to be B. orientalis specific and more sensitive than the semi-nested PCR. While previously B. orientalis had not been reported north of the Yangtse River, our results show that B. orientalis has spread to the north of the river. This could pose a serious threat to the water buffalo industry.en
dc.identifier.citationHe, L., et al., Loop-mediated isothermal amplification (LAMP) detection of Babesia He, L., et al., Loop-mediated isothermal amplification (LAMP) detection of BabesiaHe, L., et al., Loop-mediated isothermal amplification (LAMP) detection of Babesia orientalis in water buffalo (Bubalus babalis, Linnaeus, 1758) in China. Vet. Parasitol. (2009), doi:10.1016/j.vetpar.2009.06.036en
dc.identifier.issn0304-4017
dc.identifier.other10.1016/j.vetpar.2009.06.036
dc.identifier.other7004592997
dc.identifier.otherN-8706-2014
dc.identifier.urihttp://hdl.handle.net/2263/11643
dc.language.isoenen
dc.publisherElsevieren
dc.relation.requiresAdobe Acrobat Readeren
dc.rightsElsevieren
dc.subjectBabesia orientalisen
dc.subjectLoop-mediated isothermal amplification (LAMP)en
dc.subject18S rRNAen
dc.subjectBabesiosisen
dc.subject.lcshMicrobiological assayen
dc.subject.lcshBabesiosisen
dc.subject.lcshVeterinary protozoologyen
dc.subject.lcshWater buffalo (Bubalus bubalis)en
dc.subject.lcshBubalusen
dc.titleLoop-mediated isothermal amplification (LAMP) detection of Babesia orientalis in water buffalo (Bubalus babalis, Linnaeus, 1758) in Chinaen
dc.typePostprint Articleen

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
He_Loop-mediated(2009).pdf
Size:
244.73 KB
Format:
Adobe Portable Document Format
Description:
Postprint Article

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
2.43 KB
Format:
Item-specific license agreed upon to submission
Description: