Boar spermatozoa maintains DNA integrity after cryopreservation using different concentrations of glycerol

dc.contributor.authorPeña, Santiago Jr.
dc.contributor.authorGummow, Bruce
dc.contributor.authorParker, Anthony J.
dc.contributor.authorParis, Damien B.B.P.
dc.date.accessioned2024-03-14T10:04:31Z
dc.date.available2024-03-14T10:04:31Z
dc.date.issued2022-07
dc.description.abstractDespite its potential benefits, the application of frozen-thawed boar spermatozoa in commercial pig production is still very limited. Cryopreservation could induce sperm DNA damage causing reduced fertility and early embryo loss. Glycerol is a popular cryoprotectant used to improve motility and plasma membrane integrity during sperm freezing but the optimal concentration needed to prevent DNA damage without causing cell toxicity is unknown. Thus, the aim of this study was to determine the cryoprotective effect of different concentrations of glycerol on DNA integrity and motility of frozen-thawed boar spermatozoa. Using the TUNEL assay and flow cytometry, there was no significant difference in the percentage of sperm DNA damage between fresh or frozen-thawed sperm at 3%, 6%, and 8% glycerol (1.9 ± 0.4 vs. 3.5 ± 0.8 vs. 2.8 ± 0.5 vs. 3.0 ± 0.8% respectively; P > 0.05). While total and progressively motile spermatozoa were higher in fresh samples, other CASA motion parameters such as straight-line velocity, average path velocity, straightness, and linearity were generally higher in 3% and 6% glycerol-frozen than fresh samples (P ≤ 0.05). At the concentrations tested, glycerol appears to protect DNA integrity of boar spermatozoa during cryopreservation while slightly enhancing some sperm motility parameters.en_US
dc.description.departmentProduction Animal Studiesen_US
dc.description.librarianhj2024en_US
dc.description.sdgNoneen_US
dc.description.sponsorshipA JCU Development Grant, College of Public Health, Medical and Veterinary Sciences PhD Research Funds and the Australia Awards Scholarship.en_US
dc.description.urihttps://pjvm-ph.orgen_US
dc.identifier.citationPeña, S.T., Gummow, B., Parker, A.J. & Paris, D.B.B.P. 2022, 'Boar spermatozoa maintains DNA integrity after cryopreservation using different concentrations of glycerol', Philippine Journal of Veterinary Medicine, vol. 59, no. 2, pp. 164-173.en_US
dc.identifier.issn0031-7705 (print)
dc.identifier.issn2984-763X (online)
dc.identifier.urihttp://hdl.handle.net/2263/95208
dc.language.isoenen_US
dc.publisherCollege of Veterinary Medicine, University of the Philippines Los Bañosen_US
dc.rights© 2022 The Author(s). Published by UP CVM. This is an open access article under the CC BY 4.0 license.en_US
dc.subjectBoaren_US
dc.subjectPigsen_US
dc.subjectSpermen_US
dc.subjectDNA damageen_US
dc.subjectDeoxyribonucleic acid (DNA)en_US
dc.subjectGlycerolen_US
dc.subjectSperm motilityen_US
dc.subjectCryopreservationen_US
dc.subjectFreezingen_US
dc.subjectTUNELen_US
dc.subjectCASAen_US
dc.titleBoar spermatozoa maintains DNA integrity after cryopreservation using different concentrations of glycerolen_US
dc.typeArticleen_US

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