Non-destructive impedance monitoring of bacterial metabolic activity towards continuous lead biorecovery

dc.contributor.authorAndrews, George
dc.contributor.authorNeveling, Olga
dc.contributor.authorDe Beer, Dirk Johannes
dc.contributor.authorChirwa, Evans M.N.
dc.contributor.authorBrink, Hendrik Gideon
dc.contributor.authorTrudi-Heleen
dc.contributor.emailgeorge.andrews@tuks.co.zaen_US
dc.date.accessioned2023-10-16T06:55:25Z
dc.date.available2023-10-16T06:55:25Z
dc.date.issued2022-09-17
dc.descriptionDATA AVAILABILITY STATEMENT : The data used in this study are publicly available in Mendeley Data at https://DOI.org/10.17632/jfvzvwzggv.1.en_US
dc.description.abstractThe adverse health effects of the presence of lead in wastewater streams are well documented, with conventional methods of lead recovery and removal suffering from disadvantages such as high energy costs, the production of toxic sludge, and low lead selectivity. Klebsiella pneumoniae and Paraclostridium bifermentans have been identified as potential lead-precipitating species for use in a lead recovery bioreactor. Electrical impedance spectroscopy (EIS) on a low-cost device is used to determine the potential for the probe-free and label-free monitoring of cell growth in a bioreactor containing these bacteria. A complex polynomial is fit for several reactive equivalent circuit components. A direct correlation is found between the extracted supercapacitance and the plated colony-forming unit count during the exponential growth phase, and a qualitative correlation is found between all elements of the measured reactance outside the exponential growth phase. Strong evidence is found that Pb(II) ions act as an anaerobic respiration co-substrate for both cells observed, with changes in plated count qualitatively mirrored in the Pb(II) concentration. Guidance is given on the implementation of EIS devices for continuous impedance monitoring.en_US
dc.description.departmentChemical Engineeringen_US
dc.description.librarianam2023en_US
dc.description.sponsorshipThe National Research Foundation and the University of Pretoria under the Additive Manufacturing for Electronic Systems grant.en_US
dc.description.urihttps://www.mdpi.com/journal/sensorsen_US
dc.identifier.citationAndrews, G.; Neveling, O.; De Beer, D.J.; Chirwa, E.M.N., Brink, H.G.; Joubert, T.-H. Non-Destructive Impedance Monitoring of Bacterial Metabolic Activity towards Continuous Lead Biorecovery. Sensors 2022, 22, 7045. https://DOI.org/10.3390/s22187045.en_US
dc.identifier.issn1424-8220 (online)
dc.identifier.other10.3390/s22187045
dc.identifier.urihttp://hdl.handle.net/2263/92869
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rights© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en_US
dc.subjectNondestructiveen_US
dc.subjectInline monitoringen_US
dc.subjectBacterial growthen_US
dc.subjectMetabolic activityen_US
dc.subjectLead biorecoveryen_US
dc.subjectImpedance spectroscopyen_US
dc.titleNon-destructive impedance monitoring of bacterial metabolic activity towards continuous lead biorecoveryen_US
dc.typeArticleen_US

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