Molecular and phenotypic characterization of Staphylococcus aureus strains isolated from carcass swabs and carcass drips of chickens slaughtered in the informal market in Gauteng Province, South Africa

dc.contributor.authorAdigun, Oluwatola
dc.contributor.authorGcebe, Nomakorinte
dc.contributor.authorJambwa, Kudakwashe
dc.contributor.authorFasina, Folorunso Oludayo
dc.contributor.authorAdesiyun, Abiodun Adewale
dc.contributor.emailabiodun.adesiyun@up.ac.zaen_ZA
dc.date.accessioned2021-01-14T05:29:31Z
dc.date.issued2020-08
dc.descriptionAppendix 1: (Supplementary data) Frequency of resistance to antimicrobial agents by S. aureus strains from carcass swabs and dripsen_ZA
dc.description.abstractThe study was conducted to characterize Staphylococcus aureus strains from swabs and drips of dressed chicken carcasses sold at outlets in six townships in the informal market in Gauteng province, South Africa, using molecular and phenotypic methods. Seven genes (6 toxins and 1 antimicrobial resistance) comprising staphylococcal enterotoxin A (SEA), B (SEB), C (SEC), D (SED), exfoliative toxin A, toxic shock syndrome toxin, and MecA encoding methicillin resistance were assayed using polymerase chain reaction. The resistance of the S. aureus strains to 18 antimicrobial agents was determined using the disk diffusion method. The frequency of detection of the six toxin genes was sea (52.2%), followed by seb (10.9%), sec (6.5%), sed (2.2%), eta (93.5%), and tst (19.6%). The mecA gene was detected in 4.3% of the isolates. The predominant profiles of toxin genes detected were sea‐eta (37.0%). All 63 isolates of S. aureus were resistant to one or more antimicrobial agents. The frequency of resistance was high to spectinomycin (98.4%), nalidixic acid (85.7%), and penicillin (84.1%), but low to gentamycin (1.6%) and cefotaxime (1.6%). The high frequency of toxin genes and antimicrobial resistance gene observed in S. aureus isolates from chicken could pose a challenge to food safety and may have therapeutic and zoonotic implications.en_ZA
dc.description.departmentProduction Animal Studiesen_ZA
dc.description.departmentVeterinary Tropical Diseasesen_ZA
dc.description.embargo2021-04-22
dc.description.librarianhj2020en_ZA
dc.description.sponsorshipGauteng Department of Agriculture and Rural Developmenten_ZA
dc.description.urihttp://wileyonlinelibrary.com/journal/jfsen_ZA
dc.identifier.citationAdigun O, Gcebe N, Jambwa K, Fasina F, Adesiyun AA. Molecular and phenotypic characterization of Staphylococcus aureus strains isolated from carcass swabs and carcass drips of chickens slaughtered in the informal market in Gauteng Province, South Africa. Journal of Food Safety 2020;40:e12806. https://doi.org/10.1111/jfs.12806.en_ZA
dc.identifier.issn0149-6085 (print)
dc.identifier.issn1745-4565 (online)
dc.identifier.other10.1111/jfs.12806
dc.identifier.urihttp://hdl.handle.net/2263/78013
dc.language.isoenen_ZA
dc.publisherWileyen_ZA
dc.rights© 2020 Wiley Periodicals, Inc. This is the pre-peer reviewed version of the following article : Molecular and phenotypic characterization of Staphylococcus aureus strains isolated from carcass swabs and carcass drips of chickens slaughtered in the informal market in Gauteng Province, South Africa. Journal of Food Safety 2020;40:e12806. https://doi.org/10.1111/jfs.12806. The definite version is available at : http://wileyonlinelibrary.com/journal/jfs.en_ZA
dc.subjectStaphylococcus aureus strainsen_ZA
dc.subjectChickensen_ZA
dc.subjectCarcassesen_ZA
dc.subjectInformal marketen_ZA
dc.subjectGauteng Province, South Africaen_ZA
dc.titleMolecular and phenotypic characterization of Staphylococcus aureus strains isolated from carcass swabs and carcass drips of chickens slaughtered in the informal market in Gauteng Province, South Africaen_ZA
dc.typePostprint Articleen_ZA

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