Circulation of Ngari virus in livestock, Kenya

dc.contributor.authorOmoga, Dorcus Caroline Achieng
dc.contributor.authorTchouassi, David P.
dc.contributor.authorVenter, Marietjie
dc.contributor.authorOgola, Edwin O.
dc.contributor.authorEibner, Georg Joachim
dc.contributor.authorKopp, Anne
dc.contributor.authorSlothouwer, Inga
dc.contributor.authorTorto, Baldwyn
dc.contributor.authorJunglen, Sandra
dc.contributor.authorSang, Rosemary
dc.date.accessioned2023-07-24T09:08:55Z
dc.date.available2023-07-24T09:08:55Z
dc.date.issued2022-12
dc.descriptionDATA AVAILABILITY : The four isolates (KE_C166, KE_O93, KE_B02, and KE_B35) L, M, and S segments sequences were deposited in GenBank under the accession numbers ON755192 (https://www.ncbi.nlm.nih.gov/nuccore/ON755192) to ON755203 (https://www.ncbi.nlm.nih.gov/nuccore/ON755203).en_US
dc.description.abstractNgari virus (NRIV) is a mosquito-borne reassortant orthobunyavirus that causes severe febrile illness and hemorrhagic fever in humans and small ruminants. Due to limited diagnostics and surveillance, NRIV has only been detected sporadically during Rift Valley fever virus outbreaks. Little is known on its interepidemic maintenance and geographic distribution. In this study, sera from cattle, goats, and sheep were collected through a cross-sectional survey after the rainy seasons between 2020 and 2021 in two pastoralist-dominated semiarid ecosystems, Baringo and Kajiado counties in Kenya. NRIV was detected in 11 apparently healthy animals (11/2,039, 0.54%) by RT-PCR and isolated in cell culture from seven individuals. Growth analyses displayed efficient replication in cells from sheep and humans in contrast to weak replication in goat cells. NRIV infection of a wide variety of different vector cells showed only rapid replication in Aedes albopictus cells but not in cells derived from other mosquito species or sandflies. Phylogenetic analyses of complete-coding sequences of L, M, and S segments of four viruses showed that the Kenyan sequences established a monophyletic clade most closely related to a NRIV sequence from a small ruminant from Mauritania. NRIV neutralizing reactivity in cattle, goats, and sheep were 41.6% (95% CI = 30 to 54.3), 52.4% (95% CI = 37.7 to 66.6), and 19% (95% CI = 9.7 to 33.6), respectively. This is the first detection of NRIV in livestock in Kenya. Our results demonstrate active and undetected circulation of NRIV in the three most common livestock species highlighting the need for an active one-health surveillance of host networks, including humans, livestock, and vectors. IMPORTANCE : Surveillance of vectors and hosts for infection with zoonotic arthropod-borne viruses is important for early detection and intervention measures to prevent outbreaks. Here, we report the undetected circulation of Ngari virus (NRIV) in apparently healthy cattle, sheep, and goats in Kenya. NRIV is associated with outbreaks of hemorrhagic fever in humans and small ruminants. We demonstrate the isolation of infectious virus from several animals as well as presence of neutralizing antibodies in 38% of the tested animals. Our data indicate active virus circulation and endemicity likely having important implications for human and animal health.en_US
dc.description.departmentMedical Virologyen_US
dc.description.librarianhj2023en_US
dc.description.sponsorshipThe German Research Foundation (DFG); the Swedish International Development Cooperation Agency (Sida); the Swiss Agency for Development and Cooperation (SDC); the Federal Democratic Republic of Ethiopia; and the Government of the Republic of Kenya.en_US
dc.description.urihttps://journals.asm.org/journal/msphereen_US
dc.identifier.citationOmoga, D.C.A., Tchouassi, D.P., Venter, M. et al. 2022, 'Circulation of Ngari virus in livestock, Kenya', mSphere, vol. 7, no. 6, pp. 1-11, doi : 10.1128/msphere.00416-22.en_US
dc.identifier.issn2379-5042 (online)
dc.identifier.other10.1128/msphere.00416-22
dc.identifier.urihttp://hdl.handle.net/2263/91594
dc.language.isoenen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.rights© 2022 Omoga et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.en_US
dc.subjectNgari virus (NRIV)en_US
dc.subjectKenyaen_US
dc.subjectSDG-03: Good health and well-beingen_US
dc.subjectSurveillanceen_US
dc.subjectZoonotic arthropod-borne virusesen_US
dc.subjectDetectionen_US
dc.subjectInterventionen_US
dc.subjectOrthobunyavirusen_US
dc.subjectHemorrhagic feveren_US
dc.subjectLivestocken_US
dc.titleCirculation of Ngari virus in livestock, Kenyaen_US
dc.typeArticleen_US

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