Rift Valley Fever virus M and L genome segment detection : a comparison of field-deployable reverse transcription insulated isothermal PCR (RT-iiPCR) and laboratory-based multiplex reverse transcription real-time PCR

dc.contributor.authorTrujillo, Jessie D.
dc.contributor.authorWilson, William C.
dc.contributor.authorCraig, Anthony Francis
dc.contributor.authorVan den Berg, Carien
dc.contributor.authorWang, Thomas
dc.contributor.authorThompson, P.N. (Peter N.)
dc.contributor.authorSwanepoel, Robert
dc.contributor.authorMorozov, Igor
dc.contributor.authorRicht, Juergen A.
dc.date.accessioned2024-04-16T12:46:19Z
dc.date.available2024-04-16T12:46:19Z
dc.date.issued2024-03
dc.descriptionSUPPLEMENTARY MATERIAL : TABLE S1 (JCM00430-23-S0001.pdf). RVFV reference panel performance and reproducibility.en_US
dc.description.abstractRift Valley Fever phlebovirus (RVFV) is a mosquito-borne zoonotic pathogen that causes major agricultural and public health problems in Africa and the Arabian Peninsula. It is considered a potential agro-bioterrorism agent for which limited countermeasures are available. To address diagnostic needs, here we describe a rapid and sensitive molecular method immediately employable at sites of suspected outbreaks in animals that commonly precede outbreaks in humans. The strategy involves the concurrent detection of two of the three RVFV genome segments (large and medium) using reverse transcription insulated isothermal PCR (RT-iiPCR) performed on a portable, touch screen nucleic acid analyzer, POCKIT. The analytical sensitivity for both the RT-iiPCR and a laboratory-based L and M multiplex reverse transcription real-time PCR assay was estimated at approximately 0.1–3 copies/reaction using synthetic RNA or viral RNA. The diagnostic sensitivity and specificity of detection of RVFV on the POCKIT, determined using sera from sheep and cattle (n = 181) experimentally infected with two strains of RVFV (SA01 and Ken06), were 93.8% and 100% (kappa = 0.93), respectively. Testing of ruminant field sera (n = 193) in two locations in Africa demonstrated 100% diagnostic sensitivity and specificity. We conclude that the POCKIT dual-gene RVFV detection strategy can provide reliable, sensitive, and specific point-of-need viral RNA detection. Moreover, the field detection of RVFV in vectors or susceptible animal species can aid in the surveillance and epidemiological studies to better understand and control RVFV outbreaks.en_US
dc.description.departmentProduction Animal Studiesen_US
dc.description.departmentVeterinary Tropical Diseasesen_US
dc.description.librarianhj2024en_US
dc.description.sdgSDG-03:Good heatlh and well-beingen_US
dc.description.sponsorshipThe US Department of Homeland Security, Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD); the National Bio and Agro-Defense Facility (NBAF) Transition Fund from the State of Kansas; the MCB and AMP Core of the Center on Emerging and Zoonotic Infectious Diseases (CEZID) of the National Institutes of General Medical Sciences; the College of Veterinary Medicine at Iowa State University; and the Center of Advanced Host Defenses, Immunobiotics and Comparative Translational Medicine at Iowa State University.en_US
dc.description.urihttps://journals.asm.org/journal/jcmen_US
dc.identifier.citationTrujillo, J.D., Wilson, W.C., Craig, A., Van den Bergh, C., Wang, T.M., Thompson, P., Swanepoel, R., Morozov, I. & Richt, J.A. 2024. Rift Valley Fever virus M and L genome segment detection: a comparison of field-deployable reverse transcription insulated isothermal PCR (RT-iiPCR) and laboratory-based multiplex reverse transcription real-time PCR. Journal of Clinical Microbiology 62:e00430-23. https://doi.org/10.1128/jcm.00430-23.en_US
dc.identifier.issn0095-1137 (print)
dc.identifier.issn1098-660X (online)
dc.identifier.other10.1128/jcm.00430-23
dc.identifier.urihttp://hdl.handle.net/2263/95595
dc.language.isoenen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.rights© 2024 Trujillo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.en_US
dc.subjectRift Valley fever virus (RVFV)en_US
dc.subjectReverse transcription insulated isothermal PCR (RT-iiPCR)en_US
dc.subjectDetectionen_US
dc.subjectPoint-of-needen_US
dc.subjectAnimalsen_US
dc.subjectInsulated isothermal RT-PCRen_US
dc.subjectZoonosesen_US
dc.subjectSDG-03: Good health and well-beingen_US
dc.titleRift Valley Fever virus M and L genome segment detection : a comparison of field-deployable reverse transcription insulated isothermal PCR (RT-iiPCR) and laboratory-based multiplex reverse transcription real-time PCRen_US
dc.typeArticleen_US

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