Anastomosis groups and pathogenicity of Rhizoctonia solani and binucleate Rhizoctonia from potatoes in South Africa

dc.contributor.authorMuzhinji, Norman
dc.contributor.authorTruter, Mariëtte
dc.contributor.authorWoodhall, J.W.
dc.contributor.authorVan der Waals, Jacqueline Elise
dc.contributor.emailjacquie.vdwaals@up.ac.zaen_ZA
dc.date.accessioned2016-02-16T05:13:13Z
dc.date.available2016-02-16T05:13:13Z
dc.date.issued2015-12
dc.description.abstractA survey of anastomosis groups (AGs) of Rhizoctonia species associated with potato diseases was conducted in South Africa. A total of 112 Rhizoctonia solani and 19 binucleate Rhizoctonia (BNR) isolates were recovered from diseased potato plants, characterized for AG and pathogenicity. The AG identity of the isolates was confirmed using phylogenetic analysis of the internal transcribed spacer region of ribosomal DNA. Rhizoctonia solani isolates recovered belonged to AG 3-PT, AG 2- 2IIIB, AG 4HG-I, AG 4HG-III and AG 5, while BNR isolates belonged to AG A and AG R, with frequencies of 74, 6.1, 2.3, 2.3, 0.8, 12.2 and 2.3%, respectively. Rhizoctonia solani AG 3-PT was the most predominant AG and occurred in all the potato growing regions sampled whereas the other AGs occurred in distinct locations. Different AGs grouped into distinct clades with high maximum parsimony and maximum likelihood bootstrap support for both R. solani and BNR. An experiment under greenhouse conditions with representative isolates from different AGs showed differences in aggressiveness between and within AGs. Isolates of AG 2-2IIIB, AG 4HG-III and AG R were the most aggressive in causing stem canker while AG 3-PT, AG 5 and AG R caused black scurf. This is the first comprehensive survey of R. solani and BNR on potatoes in South Africa using a molecularbased approach. This is the first report of R. solani AG 2-2IIIB and AG 4 HG-I causing stem and stolon canker and BNR AG A and AG R causing stem canker and black scurf on potatoes in South Africa.en_ZA
dc.description.librarianhb2015en_ZA
dc.description.sponsorshipPotatoes South Africa.National Research Foundation of South Africa (UID: 78566 (NRF RISP grant for the ABI3500)).en_ZA
dc.description.urihttp://apsjournals.apsnet.org/loi/pdisen_ZA
dc.identifier.citationMuzhinji, N, Truter, M, Woodhall, JW & Van der Waals, JE 2015, 'Anastomosis groups and pathogenicity of Rhizoctonia solani and binucleate Rhizoctonia from potatoes in South Africa', Plant Disease, vol. 99, no. 12, pp. 1790-1802.en_ZA
dc.identifier.issn0191-2917 (print)
dc.identifier.issn1943-7692 (online)
dc.identifier.urihttp://hdl.handle.net/2263/51372
dc.language.isoenen_ZA
dc.publisherAmerican Phytopathological Societyen_ZA
dc.rights© 2015 The American Phytopathological Societyen_ZA
dc.subjectBlack scurfen_ZA
dc.subjectITS sequencesen_ZA
dc.subjectPhylogenyen_ZA
dc.subjectPathogenicityen_ZA
dc.subjectStem and stolon cankeren_ZA
dc.subjectAnastomosis groups (AGs)en_ZA
dc.subjectBinucleate Rhizoctonia (BNR)en_ZA
dc.subjectPotato diseasesen_ZA
dc.subjectSouth Africa (SA)en_ZA
dc.subjectInternal transcribed spacer (ITS)en_ZA
dc.titleAnastomosis groups and pathogenicity of Rhizoctonia solani and binucleate Rhizoctonia from potatoes in South Africaen_ZA
dc.typePreprint Articleen_ZA

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