Quantification of outcrossing events in haploid fungi using microsatellite markers

dc.contributor.authorLee, Dong Hyeon
dc.contributor.authorWingfield, Brenda D.
dc.contributor.authorRoux, Jolanda
dc.contributor.authorWingfield, Michael J.
dc.contributor.emailbrenda.wingfield@fabi.up.ac.zaen_ZA
dc.date.accessioned2020-05-05T05:57:12Z
dc.date.available2020-05-05T05:57:12Z
dc.date.issued2020-04
dc.description.abstractSpecies in genera of the fungal family Ceratocystidaceae are known to have different mating strategies, including heterothallism and homothallism. Of these, species of Ceratocystis, typified by the pathogen Ceratocystis fimbriata all undergo unidirectional mating-type switching. This implies that the pathogens possess the ability to self, but also to undergo sexual outcrossing between isolates of different mating types. In this study, we extended the recently developed microsatellite-based technique to determine the extent to which outcrossing occurs in ascospore masses of haploid fungi to two field collections of Ceratocystis albifundus. In this way, the role of reproductive strategies in shaping population structure and diversity could be better understood. Results showed that a high frequency of outcrossing occurs in isolates of the pathogen from both non-native and native areas. This explains the high level of genetic diversity previously observed in this population despite the fact that this pathogen has the ability to self.en_ZA
dc.description.departmentBiochemistryen_ZA
dc.description.departmentForestry and Agricultural Biotechnology Institute (FABI)en_ZA
dc.description.departmentGeneticsen_ZA
dc.description.departmentMicrobiology and Plant Pathologyen_ZA
dc.description.departmentPlant Production and Soil Scienceen_ZA
dc.description.librarianhj2020en_ZA
dc.description.sponsorshipThe National Research Foundation (NRF; Grant Specific Unique Reference Number, 78566, 83924), the THRIP initiative of the Department of Trade and Industry (DTI), and the Department of Trade and Industry (DST)/NRF Centre of Excellence in Tree Health Biotechnology, South Africa.en_ZA
dc.description.urihttps://www.mdpi.com/journal/jofen_ZA
dc.identifier.citationLee, D.-H.; Wingfield, B.D.; Roux, J.; Wingfield, M.J. Quantification of Outcrossing Events in Haploid Fungi Using Microsatellite Markers. J. Fungi 2020, 6, 48.en_ZA
dc.identifier.issn2309-608X (online)
dc.identifier.other10.3390/jof6020048
dc.identifier.urihttp://hdl.handle.net/2263/74469
dc.language.isoenen_ZA
dc.publisherMDPIen_ZA
dc.rights© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en_ZA
dc.subjectGenotypic diversityen_ZA
dc.subjectPopulationen_ZA
dc.subjectReproductionen_ZA
dc.subjectSexen_ZA
dc.titleQuantification of outcrossing events in haploid fungi using microsatellite markersen_ZA
dc.typeArticleen_ZA

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