Efficiencies of stationary sampling tools for the tsetse fly Glossina fuscipes fuscipes in western Kenya

dc.contributor.authorMbewe, Njelembo J.
dc.contributor.authorSole, Catherine L.
dc.contributor.authorPirk, Christian Walter Werner
dc.contributor.authorMasiga, Daniel K.
dc.contributor.authorYusuf, Abdullahi Ahmed
dc.contributor.emailcatherine.sole@up.ac.zaen_ZA
dc.date.accessioned2022-01-10T06:52:36Z
dc.date.issued2021-11
dc.description.abstractMonitoring the effectiveness of tsetse fly control interventions that aim to reduce transmission of African trypanosomiasis requires highly efficient sampling tools that can catch flies at low densities. The sticky small target (StS-target) has previously been shown to be more effective in sampling Glossina fuscipes fuscipes compared to the biconical trap. However, its efficiency in terms of the proportion of flies it catches out of those that visit it has not been reported. Furthermore, there are no reports on whether tsetse samples caught using the StS-target can be used for subsequent processes such as molecular tests. In this study, we evaluated the efficiency of the biconical trap and targets for sampling G. f. fuscipes. All targets were tiny (0.25 × 0.50 m) but varied in their capture system. We used targets with sticky surface (StS-targets) and those with an electrified surface (ES-targets). We also assessed the suitability of flies caught on the StS-target for molecular tests by amplifying DNA of bacterial communities. Randomized block design experiments were undertaken in Mbita area and Manga Island on Lake Victoria of western Kenya. Fly catches of each sampling tool were compared to those of the sampling tool flanked by electric (E) nets and analyzed using a negative binomial regression. The total catch for each sampling tool alone was divided by the total catch of the sampling tool flanked by two E-nets to obtain its efficiency expressed as a percentage. A proportion of flies caught on the StS-target was preserved for molecular tests. Overall, the efficiencies of the biconical trap, ES-target and StS-target were 7.7%, 13.3% and 27.0%, respectively. A higher proportion of females (69 to 79%) than males approached all the sampling tools, but the trap efficiency was greater for male G. f. fuscipes than females. Furthermore, sequencing the 16S rRNA gene from fly samples caught on the StS-target revealed the presence of Spiroplasma. Our results indicate that the SS-target is the most efficient trap to monitor G. f. fuscipes population during interventions, with the biconical trap being the least efficient, and samples collected from StS-targets are suitable for molecular studies.en_ZA
dc.description.departmentZoology and Entomologyen_ZA
dc.description.embargo2022-08-10
dc.description.librarianhj2021en_ZA
dc.description.sponsorshipThe European Union's integrated Biological Control Applied Research Programme (IBCARP)-tsetse repellent component; UK's Foreign, Commonwealth and Development Office (FCDO); the Swedish International Development Cooperation Agency (Sida); the Swiss Agency for Development and Cooperation (SDC); the Federal Democratic Republic of Ethiopia; and the Government of the Republic of Kenya; the University of Pretoria; the South African National Research Foundation’ IFRR.en_ZA
dc.description.urihttp://www.elsevier.com/locate/actatropicaen_ZA
dc.identifier.citationMbewe, N.J., Sole, C.L., Pirk, C.W.W. et al. 2021, 'Efficiencies of stationary sampling tools for the tsetse fly Glossina fuscipes fuscipes in western Kenya', Acta Tropica, vol. 223, art. 106092, pp. 1-7.en_ZA
dc.identifier.issn0001-706X (print)
dc.identifier.issn1873-6254 (online)
dc.identifier.other10.1016/j.actatropica.2021.106092
dc.identifier.urihttp://hdl.handle.net/2263/83109
dc.language.isoenen_ZA
dc.publisherElsevieren_ZA
dc.rights© 2021 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Acta Tropica. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Acta Tropica, vol. 223, art. 106092, pp. 1-7, 2021. doi : 10.1016/j.actatropica.2021.106092.en_ZA
dc.subjectBiconical trapen_ZA
dc.subjectES-targeten_ZA
dc.subjectStS-targeten_ZA
dc.subjectMolecular testsen_ZA
dc.subjectRiverine tsetse flyen_ZA
dc.subjectSticky small target (StS)en_ZA
dc.subjectTsetse fly (Glossina fuscipes fuscipes)en_ZA
dc.subjectKenyaen_ZA
dc.titleEfficiencies of stationary sampling tools for the tsetse fly Glossina fuscipes fuscipes in western Kenyaen_ZA
dc.typePostprint Articleen_ZA

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