The activity of Aloe arborescens Miller varieties on wound-associated pathogens, wound healing and growth factor production

dc.contributor.authorLoggenberg, Samantha Rae
dc.contributor.authorTwilley, Danielle
dc.contributor.authorDe Canha, Marco Nuno
dc.contributor.authorMeyer, Debra
dc.contributor.authorLall, Namrita
dc.contributor.emailnamrita.lall@up.ac.zaen_US
dc.date.accessioned2023-07-12T12:49:18Z
dc.date.issued2022-07
dc.description.abstractVarious Aloe L. species have been used worldwide to soothe and treat dermal wounds and burns. However, there is a lack of substantiative research on the efficacy of Aloe L. species for wound healing. The aim of this study was to investigate the differences in biological activities of the gel and ethanolic (EtOH) leaf extracts of seven Aloe arborescens Miller varieties. The extracts were investigated for their antibacterial activity against wound-associated bacteria, Staphylococcus aureus (ATCC 6538 and ATCC 25293) and Pseudomonas aeruginosa (ATCC 9027), as well as their nitric oxide (NO) scavenging potential. Varieties with antibacterial activity were further evaluated for wound closure and growth factor stimulation in human keratinocytes (HaCaT). The EtOH leaf extract of the ‘Eloff’ and ‘Jack Marais’ varieties displayed antibacterial activity against S. aureus ATCC 25293 (MIC of 500 and 250 μg/mL, respectively). The EtOH leaf extract of ‘Jack Marais’ displayed an MIC of 500 μg/mL against S. aureus ATCC 6538. The gel extract of ‘Le Roux’ exhibited antioxidant activity with a half maximal inhibitory concentration (IC50 of 2696 ± 582.66 μg/mL). The EtOH leaf extracts of ‘Eloff’ and ‘Jack Marais’ showed significant (p < 0.05) wound closure of 56.06 ± 1.47 and 73.72 ± 0.65%, respectively at 50 μg/mL and the ‘Jack Marais’ gel extract significantly stimulated wound closure (p < 0.05) by 77.02 ± 1.97 and 71.51 ± 1.11% at 50 and 100 μg/mL, respectively. Both the ‘Jack Marais’ gel extract (at 50 and 100 μg/mL) and the EtOH leaf extract (50 μg/mL), significantly (p < 0.05) increased platelet-derived growth factor (PDGF-AA) secretion to 601.09 ± 97.77, 1035.00 ± 913.98 and 559.43 ± 112.52 pg/mL, respectively. The ‘Jack Marais’ EtOH leaf extract exhibited the highest antibacterial activity, whereas the gel extract displayed the greatest potential to stimulate wound closure. This not only suggests a difference in biological activity among varieties but also between the type of extract (gel or leaf).en_US
dc.description.departmentBiochemistryen_US
dc.description.departmentGeneticsen_US
dc.description.departmentMicrobiology and Plant Pathologyen_US
dc.description.departmentPlant Production and Soil Scienceen_US
dc.description.embargo2023-06-16
dc.description.librarianhj2023en_US
dc.description.sponsorshipThe National Research Foundation of South Africa and by the Department of Science and Innovation (DSI), the Technology Innovation Agency (TIA) and South African Research Chairs Initiative (SARChI).en_US
dc.description.urihttp://www.elsevier.com/locate/sajben_US
dc.identifier.citationLoggenberg, S.R., Twilley, D., Canha, M.N.D. et al. 2022, 'The activity of Aloe arborescens Miller varieties on wound-associated pathogens, wound healing and growth factor production', South African Journal of Botany, vol. 147, pp. 1096-1104, doi : 10.1016/j.sajb.2022.04.010.en_US
dc.identifier.issn0254-6299 (print)
dc.identifier.issn1727-9321 (online)
dc.identifier.other10.1016/j.sajb.2022.04.010
dc.identifier.urihttp://hdl.handle.net/2263/91385
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rights© 2022 SAAB. Published by Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in South African Journal of Botany. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in South African Journal of Botany, vol. 147, pp. 1096-1104, 2022, doi : 10.1016/j.sajb.2022.04.010.en_US
dc.subjectWound healingen_US
dc.subjectAloe arborescencs Milleren_US
dc.subjectAntibacterialen_US
dc.subjectStaphylococcus aureusen_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjectNitric oxide scavengingen_US
dc.subjectScratch assayen_US
dc.subjectPlatelet-derived growth factor (PDGF-AA)en_US
dc.titleThe activity of Aloe arborescens Miller varieties on wound-associated pathogens, wound healing and growth factor productionen_US
dc.typePostprint Articleen_US

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