Construction and evaluation of a gfp-tagged Salmonella Typhimurium strain for environmental applications

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dc.contributor.author Burke, L.M.
dc.contributor.author Brözel, V.S. (Volker Siegfried), 1963-
dc.contributor.author Venter, S.N. (Stephanus Nicolaas)
dc.date.accessioned 2009-05-07T08:26:31Z
dc.date.available 2009-05-07T08:26:31Z
dc.date.issued 2008-01
dc.description.abstract Salmonella enterica ser. Typhimurium was isolated from freshwater sediments and chromosomally labelled with a stable variant of the green fluorescent protein (GFP). The pUT mini-Tn5 Km transposon was used to introduce the gfp gene onto the chromosome of the S. Typhimurium strain by tri-parental mating. Southern Blot hybridisation confirmed that the gene had integrated into the chromosome. The gfp gene was stably maintained and the labelled strain was not growth-rate impaired. The incorporation of the gfp gene did not convey any significant loss of phenotype which would affect the survival and behaviour of the tagged strains. The tagged S. Typhimurium strain was used to spike an established drinking water biofilm and was able to colonise and persist within the biofilm. The tagged strain was also successfully used to study the survival of S. Typhimurium in natural sediments under different temperatures. These tagged strains can therefore be used to study the fate and survival of different Salmonella strains in water environments. en
dc.identifier.citation Burke, LM, Brözel, VS & Venter, SN 2008, ‘Construction and evaluation of a gfp-tagged Salmonella Typhimurium strain for environmental applications’, Water SA, vol. 34, no. 1, pp. 19-24. [http://www.wrc.org.za/publications_watersa.htm] en
dc.identifier.issn 0378-4738
dc.identifier.uri http://hdl.handle.net/2263/9957
dc.language.iso en en
dc.publisher Water Research Council en
dc.relation.requires Adobe Acrobat en
dc.rights Water Research Council en
dc.subject Biofilm en
dc.subject Survival en
dc.subject.lcsh Salmonella typhimurium en
dc.subject.lcsh Green fluorescent protein en
dc.subject.lcsh Water-supply en
dc.title Construction and evaluation of a gfp-tagged Salmonella Typhimurium strain for environmental applications en
dc.type Article en


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