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dc.contributor.author | Korotetskiy, Ilya S.![]() |
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dc.contributor.author | Shilov, Sergey V.![]() |
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dc.contributor.author | Kuznetsova, Tatyana![]() |
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dc.contributor.author | Kerimzhanova, Bahkytzhan![]() |
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dc.contributor.author | Korotetskaya, Nadezhda![]() |
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dc.contributor.author | Ivanova, Lyudmila![]() |
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dc.contributor.author | Zubenko, Natalya![]() |
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dc.contributor.author | Parenova, Raikhan![]() |
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dc.contributor.author | Reva, Oleg N.![]() |
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dc.date.accessioned | 2024-08-27T06:14:41Z | |
dc.date.available | 2024-08-27T06:14:41Z | |
dc.date.issued | 2023-02 | |
dc.description | DATA AVAILABILITY STATEMENT : Accession numbers of DNA sequences are given in Table 1. | en_US |
dc.description | SUPPLEMENTARY INFORMATION : SUPPLEMENTARY TABLE S1: antibiotic resistance genes found in the genomes of the selected strains associated with hospital infections, SUPPLEMENTARY TABLE S2: virulence genetic determinants found in the genomes of the selected strains associated with hospital infections. | en_US |
dc.description.abstract | Hospital-acquired infections are a generally recognized problem for healthcare professionals. Clinical variants of Gram-negative and Gram-positive pathogens are characterized with enhanced antibiotic resistance and virulence due to mutations and the horizontal acquisition of respective genetic determinants. In this study, two Escherichia coli, two Klebsiella pneumoniae, three Pseudomonas aeruginosa, two Staphylococcus aureus, one Staphylococcus epidermidis and one Streptococcus pneumoniae showing broad spectra of antibiotic resistance were isolated from patients suffering from nosocomial infections in a local hospital in Almaty, Kazakhstan. The aim of the study was to compare general and species-specific pathways of the development of virulence and antibiotic resistance through opportunistic pathogens causing hospital-acquired infections. The whole-genome PacBio sequencing of the isolates allowed for the genotyping and identification of antibiotic resistance and virulence genetic determinants located in the chromosomes, plasmids and genomic islands. It was concluded that long-read sequencing is a useful tool for monitoring the epidemiological situation in hospitals. Marker antibiotic resistance mutations common for different microorganisms were identified, which were acquired due to antibiotic-selective pressure in the same clinical environment. The genotyping and identification of strain-specific DNA methylation motifs were found to be promising in estimating the risks associated with hospital infection outbreaks and monitoring the distribution and evolution of nosocomial pathogens. | en_US |
dc.description.department | Biochemistry | en_US |
dc.description.department | Genetics | en_US |
dc.description.department | Microbiology and Plant Pathology | en_US |
dc.description.librarian | am2024 | en_US |
dc.description.sdg | SDG-03:Good heatlh and well-being | en_US |
dc.description.sponsorship | The Ministry of Healthcare of the Republic of Kazakhstan. | en_US |
dc.description.uri | https://www.mdpi.com/journal/microorganisms | en_US |
dc.identifier.citation | Korotetskiy, I.S.; Shilov, S.V.; Kuznetsova, T.; Kerimzhanova, B.; Korotetskaya, N.; Ivanova, L.; Zubenko, N.; Parenova, R.; Reva, O.N. Analysis of Whole-Genome Sequences of Pathogenic Gram-Positive and Gram-Negative Isolates from the Same Hospital Environment to Investigate Common Evolutionary Trends Associated with Horizontal Gene Exchange, Mutations and DNA Methylation Patterning. Microorganisms 2023, 11, 323. https://DOI.org/10.3390/microorganisms11020323. | en_US |
dc.identifier.issn | 2076-2607 (online) | |
dc.identifier.other | 10.3390/microorganisms11020323 | |
dc.identifier.uri | http://hdl.handle.net/2263/97880 | |
dc.language.iso | en | en_US |
dc.publisher | MDPI | en_US |
dc.rights | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. | en_US |
dc.subject | Hospital infection | en_US |
dc.subject | Bacterial pathogen | en_US |
dc.subject | PacBio sequencing | en_US |
dc.subject | Genotyping | en_US |
dc.subject | Virulence factor | en_US |
dc.subject | Methylomics | en_US |
dc.subject | Deoxyribonucleic acid (DNA) | en_US |
dc.subject | Multilocus sequence typing (MLST) | en_US |
dc.subject | DNA methylation patterning | en_US |
dc.subject | SDG-03: Good health and well-being | en_US |
dc.title | Analysis of whole-genome sequences of pathogenic gram-positive and gram-negative isolates from the same hospital environment to investigate common evolutionary trends associated with horizontal gene exchange, mutations and DNA methylation patterning | en_US |
dc.type | Article | en_US |