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dc.contributor.author | Olawole, Alaba S.![]() |
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dc.contributor.author | Malahlela, M.N. (Mogaugedi)![]() |
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dc.contributor.author | Fonkui, Thierry Y.![]() |
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dc.contributor.author | Marufu, Munyaradzi Christopher![]() |
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dc.contributor.author | Cenci-Goga, Beniamino T.![]() |
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dc.contributor.author | Grispoldi, Luca![]() |
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dc.contributor.author | Etter, Eric M.C.![]() |
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dc.contributor.author | Tagwireyi, Whatmore M.![]() |
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dc.contributor.author | Karama, Musafiri![]() |
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dc.date.accessioned | 2024-08-26T09:20:54Z | |
dc.date.available | 2024-08-26T09:20:54Z | |
dc.date.issued | 2024-08 | |
dc.description | DATA AVAILABILITY : No datasets were generated or analysed during the current study. | en_US |
dc.description.abstract | Shiga toxin-producing and Enteropathogenic Escherichia coli are foodborne pathogens commonly associated with diarrheal disease in humans. This study investigated the presence of STEC and EPEC in 771 dairy cattle fecal samples which were collected from 5 abattoirs and 9 dairy farms in South Africa. STEC and EPEC were detected, isolated and identified using culture and PCR. Furthermore, 339 STEC and 136 EPEC isolates were characterized by serotype and major virulence genes including stx1, stx2, eaeA and hlyA and the presence of eaeA and bfpA in EPEC. PCR screening of bacterial sweeps which were grown from fecal samples revealed that 42.2% and 23.3% were STEC and EPEC positive, respectively. PCR serotyping of 339 STEC and 136 EPEC isolates revealed 53 different STEC and 19 EPEC serotypes, respectively. The three most frequent STEC serotypes were O82:H8, OgX18:H2, and O157:H7. Only 10% of the isolates were classified as “Top 7” STEC serotypes: O26:H2, 0.3%; O26:H11, 3.2%; O103:H8, 0.6%; and O157:H7, 5.9%. The three most frequent EPEC serotypes were O10:H2, OgN9:H28, and O26:H11. The distribution of major virulence genes among the 339 STEC isolates was as follows: stx1, 72.9%; stx2, 85.7%; eaeA, 13.6% and hlyA, 69.9%. All the 136 EPEC isolates were eaeA-positive but bfpA-negative, while 46.5% carried hlyA. This study revealed that dairy cattle are a major reservoir of STEC and EPEC in South Africa. Further comparative studies of cattle and human STEC and EPEC isolates will be needed to determine the role played by dairy cattle STEC and EPEC in the occurrence of foodborne disease in humans. | en_US |
dc.description.department | Paraclinical Sciences | en_US |
dc.description.department | Veterinary Tropical Diseases | en_US |
dc.description.librarian | hj2024 | en_US |
dc.description.sdg | SDG-02:Zero Hunger | en_US |
dc.description.sdg | SDG-03:Good heatlh and well-being | en_US |
dc.description.sponsorship | Open access funding provided by University of Pretoria. This research was funded by the National Research Foundation (NRF) of South Africa (CSRP170528234222 and SARCHI COP Grant 120317), a South African Medical Research Council Self-Initiated Research (MRC/SIR 2017–2019) grant, and the UNICEF Future Africa-UP One Health for Change Research Grants-2021. | en_US |
dc.description.uri | http://link.springer.com/journal/11274 | en_US |
dc.identifier.citation | Olawole, A.S., Malahlela, M.N., Fonkui, T.Y. et al. Occurrence, serotypes and virulence characteristics of Shiga toxin-producing and Enteropathogenic Escherichia coli isolates from dairy cattle in South Africa. World Journal of Microbiology and Biotechnology 40, 299 (2024). https://doi.org/10.1007/s11274-024-04104-w. | en_US |
dc.identifier.issn | 0959-3993 (print) | |
dc.identifier.issn | 1573-0972 (online) | |
dc.identifier.other | 10.1007/s11274-024-04104-w | |
dc.identifier.uri | http://hdl.handle.net/2263/97854 | |
dc.language.iso | en | en_US |
dc.publisher | Springer | en_US |
dc.rights | © The Author(s) 2024. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License. | en_US |
dc.subject | Dairy cattle | en_US |
dc.subject | Serotypes | en_US |
dc.subject | Virulence | en_US |
dc.subject | South Africa (SA) | en_US |
dc.subject | Shiga toxin-producing Escherichia coli (STEC) | en_US |
dc.subject | Enteropathogenic Escherichia coli (EPEC) | en_US |
dc.subject | SDG-03: Good health and well-being | en_US |
dc.subject | SDG-02: Zero hunger | en_US |
dc.title | Occurrence, serotypes and virulence characteristics of Shiga toxin-producing and Enteropathogenic Escherichia coli isolates from dairy cattle in South Africa | en_US |
dc.type | Article | en_US |