Preliminary validation of a single-spot version of a solid-phase competition ELISA for the detection of southern African territories foot-and-mouth disease serotype exposure in goats

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Authors

Gobiye, Moses
Fosgate, Geoffrey Theodore
Heath, Livio
Lazarus, David Dazhia
Seoke, LaToya
Opperman, Pamela Anne

Journal Title

Journal ISSN

Volume Title

Publisher

Elsevier

Abstract

The objective of this study was to perform a preliminary validation of a solid-phase competition ELISA (SPCE) in goats exposed to foot-and-mouth disease virus (FMDV) southern African territories (SAT) serotypes through vaccination or experimental infection. Thirty-nine goats were vaccinated with a FMDV vaccine and 37 subsequently challenged with a SAT1 virus serotype. Blood was collected every 7 days until termination at 14 days post-challenge. Single-spot SAT1 virus serotype SPCE (ss-SPCE) was performed in duplicate at two time points and a half-titration version was performed after a variable time of long-term storage. Coefficient of variation (CV) was calculated and accuracy of the ss-SPCE was estimated relative to a half-titration SPCE log10 titer of 1.6 using mixed-effect logistic regression. Additionally, sensitivity and specificity were estimated based on serological results 14-days post-challenge and at study enrolment, respectively. Three hundred and forty-two serum samples were tested in duplicate on two non-consecutive days. The median (interquartile range (IQR)) CV for the ss-SPCE for SAT1 was 2.1% (0.5, 14.3%) and 2.5% (0.6, 12.8%) for the two testing days, respectively. Median (IQR) interassay (different day) CV was 10.6% (2.5, 42.5%). Specificity and sensitivity of the ss-SPCE relative to the log10 titer using a 70% percentage inhibition positive threshold were 83.4% (95% confidence interval, 77.7–87.9) and 95.8% (90.7–98.2), respectively. Specificity was estimated as 100% (92.6, 100) and sensitivity as 97.3% (87.4, 99.9) when only considering serum tested at the beginning and end of the study, respectively. The SAT1 ss-SPCE is repeatable and accurate for determining FMDV serological status in goats.

Description

Appendix A. Supporting information

Keywords

Goats, Serology, Vaccination, Validation, Solid-phase competition ELISA (SPCE), Foot-and-mouth disease virus (FMDV), Southern African territories (SAT)

Sustainable Development Goals

None

Citation

Gobiye, M., Fosgate, G.T., Heath, L. et al. 2023, 'Preliminary validation of a single-spot version of a solid-phase competition ELISA for the detection of southern African territories foot-and-mouth disease serotype exposure in goats', Small Ruminant Research, vol. 224, art. 106982, pp. 1-9. https://DOI.org/10.1016/j.smallrumres.2023.106982.