Identification of T cell and linear B cell epitopes on African horse sickness virus serotype 4 proteins VP1-1, VP2, VP4, VP7 and NS3

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Authors

Faber, Erika
Van Schalkwyk, Antoinette
Tshilwane, Selaelo Ivy
Van Kleef, Mirinda
Pretorius, Alri

Journal Title

Journal ISSN

Volume Title

Publisher

Elsevier

Abstract

The viral proteins VP1-1, VP2, VP4, VP7 and NS3, of African horse sickness virus serotype 4 (AHSV4), have previously been identified to contain CD8+ T cell epitopes. In this study, overlapping peptides spanning the entire sequences of these AHSV4 proteins were synthesized and used to map epitopes. Peripheral blood mononuclear cells (PBMC) isolated from five horses immunized with an attenuated AHSV4 were stimulated in vitro with the synthesized peptides. Various memory immune assays were used to identify the individual peptides that contain CD8+ T cell epitopes, CD4+ T cell epitopes and linear B cell epitopes. The newly discovered individual peptides of AHSV4 proteins VP1-1, VP4, VP7 and/or NS3 that contain CD8+ T cell, CD4+ T cell or linear B cell epitopes could contribute to the design and development of new generation AHS peptide-based vaccines and therapeutics.

Description

DATA AVAILABILITY : Data will be made available on request.

Keywords

Overlapping synthetic peptides, T cell epitopes, Linear B cell epitopes, New generation AHS peptide-based vaccines, Conserved epitopes, Peripheral blood mononuclear cells (PBMC), SDG-03: Good health and well-being

Sustainable Development Goals

SDG-03:Good heatlh and well-being

Citation

Faber, E., Van Schalkwyk, A., Tshilwane, S.I. et al. 2024, 'Identification of T cell and linear B cell epitopes on African horse sickness virus serotype 4 proteins VP1-1, VP2, VP4, VP7 and NS3', Vaccine, vol. 42, no. 2, pp. 136-145, doi : 10.1016/j.vaccine.2023.12.028.