Identification of T cell and linear B cell epitopes on African horse sickness virus serotype 4 proteins VP1-1, VP2, VP4, VP7 and NS3
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Date
Authors
Faber, Erika
Van Schalkwyk, Antoinette
Tshilwane, Selaelo Ivy
Van Kleef, Mirinda
Pretorius, Alri
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
The viral proteins VP1-1, VP2, VP4, VP7 and NS3, of African horse sickness virus serotype 4 (AHSV4), have previously been identified to contain CD8+ T cell epitopes. In this study, overlapping peptides spanning the entire sequences of these AHSV4 proteins were synthesized and used to map epitopes. Peripheral blood mononuclear cells (PBMC) isolated from five horses immunized with an attenuated AHSV4 were stimulated in vitro with the synthesized peptides. Various memory immune assays were used to identify the individual peptides that contain CD8+ T cell epitopes, CD4+ T cell epitopes and linear B cell epitopes. The newly discovered individual peptides of AHSV4 proteins VP1-1, VP4, VP7 and/or NS3 that contain CD8+ T cell, CD4+ T cell or linear B cell epitopes could contribute to the design and development of new generation AHS peptide-based vaccines and therapeutics.
Description
DATA AVAILABILITY : Data will be made available on request.
Keywords
Overlapping synthetic peptides, T cell epitopes, Linear B cell epitopes, New generation AHS peptide-based vaccines, Conserved epitopes, Peripheral blood mononuclear cells (PBMC), SDG-03: Good health and well-being
Sustainable Development Goals
SDG-03:Good heatlh and well-being
Citation
Faber, E., Van Schalkwyk, A., Tshilwane, S.I. et al. 2024, 'Identification of T cell and linear B cell epitopes on African horse sickness virus serotype 4 proteins VP1-1, VP2, VP4, VP7 and NS3', Vaccine, vol. 42, no. 2, pp. 136-145, doi : 10.1016/j.vaccine.2023.12.028.